Hua Zheng-hao, Cai Song-yan, Huang Guang-jian, Wu Chun-tao, Gan Jun, Hu Cheng-en, Ni Quan-xing, Zhang Yan-ling
Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhonghua Yi Xue Za Zhi. 2008 Aug 19;88(32):2299-301.
To observe the pharmacokinetics of adriamycin-adsorbing nanometric activated carbon in intralymphatic chemotherapy.
Two ml of suspension of activated carbon with the diameter of 21 nm was mixed with adriamycin 5 mg. Eighteen dogs were randomly divided into 6 equal groups. The above mentioned mixture was injected subserosally to the anterior wall of gastric antrum of the dogs. Thirty minutes, 1 h, 2 h, 1 day, and 3 days after the injection the gastroepiploic lymph nodes of the Groups 1 - 5 were obtained. And Group 6 underwent extraction of venous blood samples 5, 15, 30, 60, 120, and 240 minutes after the injection and extraction of thoracic duct fluid 5, 15, 30, 60, 120, 240, and 360 minutes after the injection. The adriamycin concentrations at different time points were determined by mass spectrometer. The lymphatic vessels and nodes at the gastric wall were observed by the naked eyes.
Black tiny lymphatic vessels and lymph nodes were visualized around the injection areas immediately after the injection. Adriamycin content could be detected 30 min after the injection and lasted for 72 h at high levels with the peak content of (84.6 +/- 2.0) microg per gram tissue at 60 min in the perilymph node of gastroepiploic artery. The adriamycin concentration in the lymph fluid of thoracic duct reached the top level of 162.5 ng/ml 30 min after the injection, and then decreased slowly. Adriamycin could be still detected in lymph fluid 6 h after injection. No trace of adriamycin was found in the blood at any time points.
The content of adriamycin can keep high and last long in the drainage of lymph node and lymph fluid in the treatment of intralymphatic chemotherapy using adriamycin-adsorbing nanometric activated carbon.
观察阿霉素吸附纳米活性炭在淋巴化疗中的药代动力学。
将2ml直径为21nm的活性炭混悬液与5mg阿霉素混合。18只犬随机分为6组，每组3只。将上述混合物浆膜下注射到犬胃窦前壁。注射后30分钟、1小时、2小时、1天和3天，获取第1 - 5组犬的胃网膜淋巴结。第6组在注射后5、15、30、60、120和240分钟采集静脉血样本，并在注射后5、15、30、60、120、240和360分钟采集胸导管液。用质谱仪测定不同时间点的阿霉素浓度。肉眼观察胃壁的淋巴管和淋巴结。
注射后立即在注射部位周围可见黑色微小淋巴管和淋巴结。注射后30分钟可检测到阿霉素含量，高水平持续72小时，胃网膜动脉周围淋巴结在60分钟时阿霉素含量峰值为（84.6±2.0）μg/g组织。胸导管淋巴液中阿霉素浓度在注射后30分钟达到最高水平162.5ng/ml，然后缓慢下降。注射后6小时仍可在淋巴液中检测到阿霉素。在任何时间点的血液中均未发现阿霉素痕迹。
在使用阿霉素吸附纳米活性炭进行淋巴化疗时，阿霉素在淋巴结和淋巴液引流中的含量可保持较高水平且持续时间长。
