卤虫胚胎的延伸因子2及其与核糖体的亲和力。

Elongation factor 2 from Artemia salina embryos and its affinity for ribosomes.

作者信息

Zamboni M, Brigotti M, Montanaro L, Sperti S

机构信息

Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.

出版信息

Eur J Biochem. 1991 Aug 15;200(1):13-8. doi: 10.1111/j.1432-1033.1991.tb21042.x.

DOI:10.1111/j.1432-1033.1991.tb21042.x

PMID:1908776

Abstract

Crude extracts from Artemia salina undeveloped embryos do not contain detectable elongation-factor-2 (EF2) kinase and endogenous ADP-ribosylating activities. Accordingly, EF2 purified from this source is an enzyme relatively free from phosphorylated and ADP-ribosylated forms. Endogenous ADP-ribosyltransferase activity appears only after purification of EF2. The affinities of EF2 and of ADP-ribosyl-EF2 for ribosomes from A. salina undeveloped embryos have been calculated by measuring the ability of the factors to inhibit the N-glycosidase activity of ricin on ribosomes.

摘要

卤虫未发育胚胎的粗提物中未检测到可检测的延伸因子2（EF2）激酶和内源性ADP核糖基化活性。因此，从该来源纯化的EF2是一种相对不含磷酸化和ADP核糖基化形式的酶。内源性ADP核糖基转移酶活性仅在EF2纯化后出现。通过测量这些因子抑制蓖麻毒素对卤虫未发育胚胎核糖体的N-糖苷酶活性的能力，计算了EF2和ADP核糖基-EF2对卤虫未发育胚胎核糖体的亲和力。