Satoh M, Tanaka T, Kushiro A, Hakoshima T, Tomita K
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
FEBS Lett. 1991 Aug 19;288(1-2):98-100. doi: 10.1016/0014-5793(91)81011-v.
The tufB gene encoding elongation factor Tu (EF-Tu) of Thermus thermophilus HB8 was cloned and expressed. Compared with the known tufA gene of T. thermophilus, nucleotide differences were found at 10 positions out of 1221 nucleotides, and amino acid substitutions were found at 4 positions out of 406 amino acids. The tufB product was 70.9% homologous to the corresponding sequence of the tufB product of E. coli. The G+C content of the third base of the codon in the tufB gene was 84.8% and G was especially preferred in this position.
克隆并表达了嗜热栖热菌HB8编码延伸因子Tu（EF-Tu）的tufB基因。与嗜热栖热菌已知的tufA基因相比，在1221个核苷酸中的10个位置发现了核苷酸差异，在406个氨基酸中的4个位置发现了氨基酸替换。tufB产物与大肠杆菌tufB产物的相应序列同源性为70.9%。tufB基因中密码子第三个碱基的G+C含量为84.8%，且该位置特别偏好G。
