Goebel W, Chakraborty T, Domann E, Köhler S, Kuhn M, Leimeister-Wächter M, Sokolovic Z, Wuenscher M
Institut für Genetik und Mikrobiologie, Würzburg, Germany.
Infection. 1991;19 Suppl 4:S195-7. doi: 10.1007/BF01644032.
The characterization of mutants of Listeria monocytogenes with reduced virulence properties is described. Reduction in the amount of the extracellular protein p60 (encoded by the ipa gene) leads to cell filaments with impaired invasiveness. Mutants which cannot synthesize listeriolysin are still invasive but unable to survive within phagocytic cells. One type of listeriolysin-negative mutants is defective in the synthesis of a positive regulatory element PrfA which coordinately regulates the listeriolysin gene (lisA) together with several other genes, including those for a phosphatidylinositol-specific phospholipase and a metalloprotease.
本文描述了毒力特性降低的单核细胞增生李斯特菌突变体的特征。细胞外蛋白p60（由ipa基因编码）数量的减少会导致侵袭性受损的细胞丝。无法合成李斯特菌溶血素的突变体仍具有侵袭性，但无法在吞噬细胞内存活。一种李斯特菌溶血素阴性突变体在合成正调控元件PrfA方面存在缺陷，PrfA与包括磷脂酰肌醇特异性磷脂酶和金属蛋白酶基因在内的其他几个基因共同协调调控李斯特菌溶血素基因（lisA）。
