Ono-Ogasawara Mariko, Myojo Toshihiko, Smith Thomas J
National Institute of Occupational Safety and Health, Kawasaki, Japan.
Ind Health. 2008 Dec;46(6):582-93. doi: 10.2486/indhealth.46.582.
A simple direct injection method for analysis of polycyclic aromatic hydrocarbons (PAHs) or other organic components of particulate matter by gas chromatography/mass spectrometry (GC/MS) was developed. This method uses a small custom brass capsule to insert a particulate sample deposited on a quartz fiber filter or powdery sample directly into a GC injector inlet. This approach was applied in the analysis of PAHs in diesel emissions and other particulate samples, which are introduced into the GC and analyzed by MS using selected ion monitoring mode. Quantitation is based on the concentration of PAHs in a standard reference material (SRM), which are certified by US National Institute of Standard Technology to avoid matrix effects on extraction efficiency. A standard filter is prepared by deposition of small amount of SRM of diesel particulate or ambient PM on quartz fiber filter. By this method, the linearity for 12 kinds of PAHs (3 to 6 rings) was demonstrated from 0.022 mg to 0.65 mg of SRM 1650. The linearity of BaP, for example, was confirmed from 0.028 to 0.845 ng. The reproducibility of this method determined by analyzing the standard filter was 15.4%. By selecting an appropriate SRM as a standard material, this method is applicable for analyzing PAHs in fine particulate matter of less than 1 microm from various origins. Preliminary results for a series of ambient particulate matter, roadside PM, diesel soot and sidestream smoke, are presented. This method enables analysis of organic chemical substances, for example PAHs, in PM without any pretreatment using organic solvent, and without any expensive modification of GC instrument.
开发了一种通过气相色谱/质谱联用仪（GC/MS）分析多环芳烃（PAHs）或颗粒物中其他有机成分的简单直接进样方法。该方法使用一个小型定制黄铜胶囊，将沉积在石英纤维滤膜上的颗粒物样品或粉末状样品直接插入GC进样口。此方法应用于柴油排放物和其他颗粒物样品中PAHs的分析，这些样品被引入GC并采用选择离子监测模式通过MS进行分析。定量基于标准参考物质（SRM）中PAHs的浓度，该标准参考物质由美国国家标准技术研究所认证，以避免基质对萃取效率的影响。通过在石英纤维滤膜上沉积少量柴油颗粒物或环境颗粒物的SRM来制备标准滤膜。通过该方法，证明了12种PAHs（3至6环）在0.022 mg至0.65 mg的SRM 1650范围内具有线性关系。例如，BaP的线性关系在0.028至0.845 ng范围内得到确认。通过分析标准滤膜确定该方法的重现性为15.4%。通过选择合适的SRM作为标准物质，该方法适用于分析来自各种来源的粒径小于1微米的细颗粒物中的PAHs。给出了一系列环境颗粒物、路边颗粒物、柴油烟灰和侧流烟气的初步结果。该方法能够在不使用有机溶剂进行任何预处理且不对GC仪器进行任何昂贵改装的情况下，分析颗粒物中的有机化学物质，例如PAHs。
