Li Zhi Qiang, Duan Li Ping, Shen Li
Department of Gastroenterology, Peking University Third Hospital, Beijing, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2008 Dec 18;40(6):624-8.
To investigate the differentiation of human neural stem cells (hNSCs) in vitro and to assess the potential of hNSCs transplanted into the pylorus of nude mice.
Central nervous system-derived hNSCs (CNS-hNSCs) were obtained from the subventricular zone of aborted fetus brain (10 weeks), suspension cultured, passaged and induced to differentiate in vitro. Stem cell marker neuroepithelial stem cell protein (nestin), enteric neuronal marker protein gene production 9.5 (PGP9.5) and glial cell marker glial fibrillary acidic protein (GFAP) were detected by immunofluorescence method. CNS-hNSCs (cultured for 3 months) were transplanted into the pylorus of nude mice and followed by immunofluorescence method.
Using the immunofluorescence method, nestin could be identified and the induced cells expressed PGP9.5 and GFAP. After being transplanted into the pylorus of nude mice, CNS-hNSC could be detected to survive at least for 6 weeks.
CNS-hNSCs could be cultured, passaged and induced in vitro successfully; transplantation of CNS-hNSCs into gastrointestinal tract could be a potential cellular replacement strategy for gastrointestinal motility disorders.
研究人神经干细胞（hNSCs）在体外的分化情况，并评估hNSCs移植到裸鼠幽门的潜力。
从流产胎儿（10周）脑的脑室下区获取中枢神经系统来源的hNSCs（CNS-hNSCs），进行悬浮培养、传代并在体外诱导分化。采用免疫荧光法检测干细胞标志物神经上皮干细胞蛋白（巢蛋白）、肠神经元标志物蛋白基因产物9.5（PGP9.5）和胶质细胞标志物胶质纤维酸性蛋白（GFAP）。将培养3个月的CNS-hNSCs移植到裸鼠幽门，然后采用免疫荧光法进行观察。
采用免疫荧光法可鉴定出巢蛋白，诱导后的细胞表达PGP9.5和GFAP。将CNS-hNSCs移植到裸鼠幽门后，可检测到其至少存活6周。
CNS-hNSCs可在体外成功培养、传代和诱导；将CNS-hNSCs移植到胃肠道可能是治疗胃肠动力障碍的一种潜在细胞替代策略。
