[Study on human neural stem cells differentiation in vitro and transplantation in nude mice].

OBJECTIVE

To investigate the differentiation of human neural stem cells (hNSCs) in vitro and to assess the potential of hNSCs transplanted into the pylorus of nude mice.

METHODS

Central nervous system-derived hNSCs (CNS-hNSCs) were obtained from the subventricular zone of aborted fetus brain (10 weeks), suspension cultured, passaged and induced to differentiate in vitro. Stem cell marker neuroepithelial stem cell protein (nestin), enteric neuronal marker protein gene production 9.5 (PGP9.5) and glial cell marker glial fibrillary acidic protein (GFAP) were detected by immunofluorescence method. CNS-hNSCs (cultured for 3 months) were transplanted into the pylorus of nude mice and followed by immunofluorescence method.

RESULTS

Using the immunofluorescence method, nestin could be identified and the induced cells expressed PGP9.5 and GFAP. After being transplanted into the pylorus of nude mice, CNS-hNSC could be detected to survive at least for 6 weeks.

CONCLUSION

CNS-hNSCs could be cultured, passaged and induced in vitro successfully; transplantation of CNS-hNSCs into gastrointestinal tract could be a potential cellular replacement strategy for gastrointestinal motility disorders.