Wu Chia-Chang, Chu Chun, Wang Yei-Shung, Lur Huu-Sheng
Tea Research Extension Station, Nantou, Taiwan.
J Environ Sci Health B. 2009 Jan;44(1):58-68. doi: 10.1080/03601230802519744.
A multiresidue method was developed to determine 19 carbamate pesticides in tea samples. Optimizations of different parameters, such as the type of extraction solvents, clean-up cartridges, and elution solvents were carried out. The developed method used acetonitrile as extraction solvent, amino cartridge for adsorbents and acetone-n-hexane as the eluting solution. Nineteen carbamate residues were then analyzed by high-pressure liquid chromatography (HPLC) with fluorescence detector. The present results showed good linearity by correlation coefficients of more than 0.9999 for all analyses. Limits of detection and quantification varied from 0.0005-0.023 mg L(- 1), 0.008-0.077 mg L(- 1), respectively. Recoveries of sixteen carbamate pesticides ranged from 65% to 135% at the spiked level of 0.5, 1.0 and 2.0 mg L(- 1). The relative standard deviations were lower than 20% and coefficient of variations were lower than 15%. The results indicate that the proposed method provides an effective multi and trace level screening determination of carbamate pesticides residues for tea samples.
建立了一种多残留方法来测定茶叶样品中的19种氨基甲酸酯类农药。对不同参数进行了优化，如萃取溶剂的类型、净化柱和洗脱溶剂。所建立的方法使用乙腈作为萃取溶剂，氨基柱作为吸附剂，丙酮 - 正己烷作为洗脱液。然后用带荧光检测器的高压液相色谱（HPLC）分析19种氨基甲酸酯类残留。目前的结果表明，所有分析的相关系数均大于0.9999，线性良好。检测限和定量限分别为0.0005 - 0.023 mg L(-1)、0.008 - 0.077 mg L(-1)。在0.5、1.0和2.0 mg L(-1)的加标水平下，16种氨基甲酸酯类农药的回收率在65%至135%之间。相对标准偏差低于20%，变异系数低于15%。结果表明，该方法为茶叶样品中氨基甲酸酯类农药残留提供了一种有效的多残留和痕量水平筛查测定方法。
