Hu Xiuli, Chen Xuesi, Wei Jizheng, Liu Shi, Jing Xiabin
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Macromol Biosci. 2009 May 13;9(5):456-63. doi: 10.1002/mabi.200800158.
A biodegradable amphiphilic block copolymer, PEG-b-P(LA-co-MAC), was used to prepare spherical micelles consisting of a hydrophobic P(LA-co-MAC) core and a hydrophilic PEG shell. To improve their stability, the micelles were crosslinked by radical polymerization of the double bonds in the hydrophobic blocks. The crosslinked micelles had similar sizes and a narrow size distribution compared to their uncrosslinked precursor. The improved stability of the crosslinked micelles was confirmed by measurements of the CMC and a thermodynamic investigation. These micelles can internalize into Hela cells in vitro as demonstrated by inverted fluorescence microscopy and CLSM. These stabilized nanoscale micelles have potential use in biomedical applications such as drug delivery and disease diagnosis.
一种可生物降解的两亲性嵌段共聚物PEG-b-P(LA-co-MAC)被用于制备由疏水性P(LA-co-MAC)核和亲水性PEG壳组成的球形胶束。为提高其稳定性，通过疏水嵌段中双键的自由基聚合对胶束进行交联。与未交联的前体相比，交联胶束具有相似的尺寸和较窄的尺寸分布。通过临界胶束浓度测量和热力学研究证实了交联胶束稳定性的提高。如倒置荧光显微镜和共聚焦激光扫描显微镜所示，这些胶束能够在体外内化进入Hela细胞。这些稳定的纳米级胶束在药物递送和疾病诊断等生物医学应用中具有潜在用途。
