Fazan Valéria Paula Sassoli, Rodrigues Filho Omar Andrade, Jordão Caroline Echavarria Rodrigues, Moore Kenneth Charles
Department of Surgery and Anatomy, School of Medicine of Ribeirão Preto, University of São Paulo, Sao Paulo, Brazil.
Anat Rec (Hoboken). 2009 Apr;292(4):513-7. doi: 10.1002/ar.20843.
Despite numerous literature reports on the morphometry of the myelinated fibers of phrenic nerves in rats, a systematic study of the longitudinal and lateral symmetry of the unmyelinated fibers morphometry is not available. In this study, we have undertaken ultrastructural and morphometric studies of the phrenic nerve in adult rats, assessing two different levels (proximal and distal) from both right and left sides. Phrenic nerves of adult male Wistar rats were prepared for epoxy resin embedding and transmission electron microscopy. Morphometric analysis was performed with the aid of computer software, which took into consideration the unmyelinated fiber number, density, area, and diameter, as well as ratio between myelinated and unmyelinated fibers, and the percentage of the fascicular area occupied by the myelinated and unmyelinated fibers. Comparison of data from proximal and distal segments on the same side and from the same levels between sides was performed. Differences were considered significant when P < 0.05. The most important finding is that morphometric parameters of the phrenic nerve unmyelinated fibers in adult rats are both longitudinally and laterally symmetric. This study adds important morphometric information about the unmyelinated fibers of the phrenic nerves in adult rats for proximal and distal levels on both sides of the animal.
尽管有大量关于大鼠膈神经有髓纤维形态计量学的文献报道，但关于无髓纤维形态计量学的纵向和横向对称性的系统研究却未见报道。在本研究中，我们对成年大鼠的膈神经进行了超微结构和形态计量学研究，评估了左右两侧的两个不同水平（近端和远端）。将成年雄性Wistar大鼠的膈神经制备用于环氧树脂包埋和透射电子显微镜检查。借助计算机软件进行形态计量分析，该软件考虑了无髓纤维的数量、密度、面积和直径，以及有髓和无髓纤维之间的比例，还有有髓和无髓纤维所占束状区域的百分比。对同一侧近端和远端节段的数据以及两侧相同水平的数据进行了比较。当P < 0.05时，差异被认为具有显著性。最重要的发现是成年大鼠膈神经无髓纤维的形态计量参数在纵向和横向上都是对称的。本研究为动物两侧近端和远端水平的成年大鼠膈神经无髓纤维增加了重要的形态计量学信息。
