Farkhari M, Naghavi M R, Pyghambari S A
Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran.
Pak J Biol Sci. 2007 Sep 1;10(17):2868-73. doi: 10.3923/pjbs.2007.2868.2873.
Genetic variation of 28 populations of jointed goatgrass (Aegilops cylindrica Host.), collected from different parts of Iran, were evaluated using both RAPD-PCR and SDS-PAGE of seed proteins. The diversity within and between populations for the three-band High Molecular Weight (HMW) subunits of glutenin pattern were extremely low. Out of 15 screened primers of RAPD, 14 primers generated 133 reproducible fragments which among them 92 fragments were polymorphic (69%). Genetic similarity calculated from the RAPD data ranged from 0.64 to 0.98. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm and separated the 28 populations into two groups. Confusion can happen between populations with the same origin as well as between populations of very diverse geographical origins. Our results show that compare to seed storage protein, RAPD is suitable for genetic diversity assessment in Ae. cylindrica populations.
利用随机扩增多态性DNA聚合酶链式反应（RAPD-PCR）和种子蛋白的十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）技术，对从伊朗不同地区采集的28个节节麦（Aegilops cylindrica Host.）种群的遗传变异进行了评估。谷蛋白模式的三条带高分子量（HMW）亚基在种群内和种群间的多样性极低。在15条筛选的RAPD引物中，14条引物产生了133个可重复片段，其中92个片段具有多态性（69%）。根据RAPD数据计算的遗传相似性范围为0.64至0.98。基于相似性矩阵，使用非加权组平均法（UPGMA）算法绘制了一个聚类图，将28个种群分为两组。同一来源的种群之间以及地理来源非常不同的种群之间都可能出现混淆。我们的结果表明，与种子贮藏蛋白相比，RAPD适用于节节麦种群遗传多样性的评估。
