甘蔗线条花叶病毒重组外壳蛋白组装成类马铃薯Y病毒颗粒。
Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles.
作者信息
Hema M, Subba Reddy Ch V, Savithri H S, Sreenivasulu P
机构信息
Department of Virology, College of Biological and Earth Sciences, Sri Venkateswara University, Tirupati 517 502, India.
出版信息
Indian J Exp Biol. 2008 Nov;46(11):793-6.
Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.
甘蔗条纹花叶病毒AP分离株（SCSMV-AP）的外壳蛋白（CP）基因在大肠杆菌中表达，重组CP（SCSMV-AP rCP）通过线性蔗糖密度梯度离心法进行纯化。在电子显微镜下观察纯化后的SCSMV-AP rCP，发现存在类马铃薯Y病毒颗粒（PVLPs）。通过狭缝杂交显示，组装的颗粒能够包裹CP基因转录本。
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