Paulke-Korinek Maria, Rendi-Wagner Pamela, Kundi Michael, Tomann Batya, Wiedermann Ursula, Kollaritsch Herwig
Department of Specific Prophylaxis and Tropical Medicine, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria.
J Travel Med. 2008 Nov-Dec;15(6):437-41. doi: 10.1111/j.1708-8305.2008.00252.x.
When deciding whether to administer a tetanus vaccination--for international travel or injury--a subject's vaccination certificate should be investigated. As many people lack valid vaccination cards and are unable to recall their vaccination history, the Tetanos Quick Stick (TQS) test rapidly detects protective tetanus immunoglobulin IgG antibodies in whole blood, serum, or plasma. This immunochromatographic dipstick test yields a positive or negative result.
Our study evaluates the effectiveness of the TQS test by comparing the binary TQS test results of 100 sera with the tetanus antibody levels as measured by the standardized enzyme-linked immunosorbent assay (ELISA) method. We used the TQS test to determine whether a person needed a tetanus booster vaccination. If the test showed a clearly visible line that was similar to the control line, the result was determined to be positive.
All positive TQS test results had a concentration of IgG antibodies above 0.5 IU/mL as measured by ELISA, indicating that no booster vaccination was required. Similarly, in all cases with an antibody level below 0.1 IU/mL, where a vaccination would have been recommended based on the ELISA test result, the TQS test yielded a negative result. The positive predictive value and the specificity for the dipstick test were therefore 100%.
The TQS test is a reliable, fast, and cost-effective means of identifying subjects with a preexisiting level of tetanus IgG antibodies above approximately 0.5 IU/mL. This can help to avoid unnecessary tetanus vaccinations in travel clinics, emergency departments, and practices of family doctors.
在决定是否进行破伤风疫苗接种时（无论是出于国际旅行还是受伤原因），应查看受试者的疫苗接种证明。由于许多人没有有效的疫苗接种卡且无法回忆起他们的疫苗接种史，破伤风快速检测棒（TQS）测试可快速检测全血、血清或血浆中具有保护性的破伤风免疫球蛋白IgG抗体。这种免疫层析试纸条测试会得出阳性或阴性结果。
我们的研究通过将100份血清的二元TQS测试结果与采用标准化酶联免疫吸附测定（ELISA）方法测得的破伤风抗体水平进行比较，来评估TQS测试的有效性。我们使用TQS测试来确定一个人是否需要接种破伤风加强疫苗。如果测试显示出一条与对照线相似的清晰可见的线，则结果判定为阳性。
所有TQS测试阳性结果的IgG抗体浓度经ELISA测定均高于0.5 IU/mL，这表明无需接种加强疫苗。同样，在所有抗体水平低于0.1 IU/mL的病例中，根据ELISA测试结果本应建议接种疫苗，而TQS测试均得出阴性结果。因此，试纸条测试的阳性预测值和特异性均为100%。
TQS测试是一种可靠、快速且经济高效的方法，可用于识别破伤风IgG抗体预先存在水平高于约0.5 IU/mL的受试者。这有助于在旅行诊所、急诊科和家庭医生诊所避免不必要的破伤风疫苗接种。
