Riske F J, Cullen B R, Chizzonite R
Department of Molecular Genetics, Roche Research Center, Hoffman-La Roche Inc., Nutley, NJ 07110.
Lymphokine Cytokine Res. 1991 Jun;10(3):213-8.
Human interleukin-2 (IL-2) and human interferon-gamma (IF-gamma) isolated from transfected heterologous cell lines were structurally compared with their natural counterparts isolated from peripheral blood lymphocytes (PBL). Several forms of IL-2 and IFN-gamma that were primarily due to differential glycosylation were produced by all cell types studied. Comparable molecular weights and sugar compositions were found for variants of natural and mammalian-derived recombinant IL-2, and variants of natural and mammalian-derived recombinant IFN-gamma. Furthermore, the specific biological activities of the natural and mammalian-derived recombinant lymphokines were similar and comparable to the specific activity of the Escherichia coli-derived recombinant proteins.
将从转染的异源细胞系中分离出的人白细胞介素-2（IL-2）和人干扰素-γ（IF-γ），与从外周血淋巴细胞（PBL）中分离出的天然对应物进行结构比较。所有研究的细胞类型都产生了几种主要因糖基化差异而形成的IL-2和IF-γ形式。天然来源和哺乳动物来源的重组IL-2变体，以及天然来源和哺乳动物来源的重组IF-γ变体，具有相当的分子量和糖组成。此外，天然来源和哺乳动物来源的重组淋巴因子的比生物活性相似，且与大肠杆菌来源的重组蛋白的比活性相当。
