Dusane D H, Nancharaiah Y V, Venugopalan V P, Kumar A R, Zinjarde S S
Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
Water Sci Technol. 2008;58(12):2467-75. doi: 10.2166/wst.2008.585.
Biofilm formation by Yarrowia lipolytica, a biotechnologically important fungus in microtitre plates, on glass slide surfaces and in flow cell was investigated. In microtitre plates, there was a short lag phase of adhesion followed by a period of rapid biofilm growth. The fungus formed extensive biofilms on glass slides, whereas in flow-cells a multicellular, three-dimensional microcolony structure was observed. The isolate formed biofilms in seawater and in fresh water media at neutral pH when grown in microtitre plates. The carbon sources differentially affected formation of biofilms in microtitre plates. Lactic acid, erythritol, glycerol, glucose and edible oils supported the formation of biofilms, while alkanes resulted in sub-optimal biofilm development. A variation in the morphology of the fungus was observed with different carbon sources. The results point to the possible existence of highly structured biofilms in varied ecological niches from where the yeast is isolated.
研究了解脂耶氏酵母（一种在生物技术领域具有重要意义的真菌）在微量滴定板、载玻片表面和流动池中形成生物膜的情况。在微量滴定板中，存在一个短暂的粘附滞后期，随后是生物膜快速生长的阶段。该真菌在载玻片上形成了广泛的生物膜，而在流动池中观察到了多细胞三维微菌落结构。当在微量滴定板中培养时，该分离株在中性pH值的海水和淡水培养基中形成生物膜。碳源对微量滴定板中生物膜的形成有不同影响。乳酸、赤藓糖醇、甘油、葡萄糖和食用油促进生物膜的形成，而烷烃导致生物膜发育不理想。观察到该真菌在不同碳源下形态有所变化。结果表明，从分离酵母的不同生态位可能存在高度结构化的生物膜。
