Hashemi Seyed Mahmoud, Soleimani Masoud, Zargarian Seyed Shahrooz, Haddadi-Asl Vahid, Ahmadbeigi Naser, Soudi Sara, Gheisari Yousof, Hajarizadeh Athena, Mohammadi Yousef
Department of Stem Cell and Tissue Engineering, Stem Cell Technology Co. Ltd., Tehran, Iran.
Cells Tissues Organs. 2009;190(3):135-49. doi: 10.1159/000187716. Epub 2008 Dec 18.
Tissue engineering of implantable cellular constructs is an emerging cellular therapy for hepatic disease. In this study, we tested the ability of poly(epsilon-caprolactone) (PCL) nanofiber scaffold to support and maintain hepatic differentiation of human cord blood-derived unrestricted somatic stem cells (USSCs) in vitro. USSCs, self-renewing pluripotent cells, were isolated from human cord blood. The electrospun PCL nanofiber porous scaffold was constructed of uniform, randomly oriented nanofibers. USSCs were seeded onto PCL nanofiber scaffolds, and were induced to differentiate into hepatogenic lineages by culturing with differentiation factors for 6 weeks. RT-PCR analysis of endoderm and hepatic-specific gene expression, immunohistochemical detection of cytokeratin 18 (CK-18), alpha-fetoprotein, albumin, glycogen storage and indocyanine green uptake confirmed the differentiation of USSCs into endoderm and hepatocyte-like cells. In the present study, we show that hepatocyte-like cells differentiated from USSCs on the PCL nanofiber scaffold can be candidate for tissue engineering and cell therapy of hepatic tissues.
可植入细胞构建体的组织工程是一种新兴的肝脏疾病细胞治疗方法。在本研究中，我们测试了聚（ε-己内酯）（PCL）纳米纤维支架在体外支持和维持人脐带血来源的无限制体细胞干细胞（USSCs）肝分化的能力。USSCs是从人脐带血中分离出来的自我更新多能细胞。静电纺丝PCL纳米纤维多孔支架由均匀、随机取向的纳米纤维构成。将USSCs接种到PCL纳米纤维支架上，并通过与分化因子培养6周诱导其分化为肝源性谱系。对内胚层和肝脏特异性基因表达的RT-PCR分析、细胞角蛋白18（CK-18）、甲胎蛋白、白蛋白、糖原储存和吲哚菁绿摄取的免疫组织化学检测证实了USSCs向内胚层和肝细胞样细胞的分化。在本研究中，我们表明在PCL纳米纤维支架上从USSCs分化而来的肝细胞样细胞可成为肝组织组织工程和细胞治疗的候选细胞。
