[转化生长因子β1对食管鳞状细胞癌上皮-间质转化的调控]

[Transforming growth factor beta1 regulation of epithelial-mesenchymal transition in esophagus squamous cell carcinoma].

作者信息

Sun Yang, Li Shan-shan, Wang Xin-hua, Wang Xiao-jun, Yan Ai-hua

机构信息

Department of Pathology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2008 Aug;37(8):542-8.

PMID:19094466

Abstract

OBJECTIVE

To study the functional role of transforming growth factor beta1(TGFbeta1) in the regulation of epithelial-mesenchymal transition (EMT) and the effect of TGFbeta1-ASODN blockage of EMT in esophagus squamous cell carcinoma.

METHODS

Esophageal squamous cell carcinoma cell line EC9706 was transfected with chemically synthesized TGFbeta1-ASODN. RT-PCR, immunohistochemistry and flow cytometry were used to detect the protein and mRNA expressions of TGF-beta1, E-cadherin and vimentin before and after the transfection. Morphological changes were documented and scarification test was used to detect the migration potential of EC9706 before and after the transfection.

RESULTS

After TGFbeta1-ASODN transfection, mRNA (0.25 +/- 0.07) and protein (35.07% +/- 1.42%) expressions of TGFbeta1 in EC9706 were significantly lower than those before transfection (mRNA: 0.43 +/- 0.09; protein: 43.57% +/- 1.77%, chi(2) = 13.847 and chi(2) = 84.120, P < 0.05). The mRNA (0.38 +/- 0.09) and protein (17.13% +/- 1.45%) expressions of E-cadherin were significantly higher than those before transfection (0.22 +/- 0.06; 12.53% +/- 1.31%, chi(2) = 0.160 and chi(2) = 40.008, P < 0.05) and the mRNA (0.73 +/- 0.07) and protein (14.15% +/- 1.46%) expressions of vimentin were significantly lower than those (0.89 +/- 0.09; 17.97% +/- 1.42%) before transfection (chi(2) = 0.160 and chi(2) = 21.103, P < 0.05). Scarification test showed that after transfection, the mobility of EC9706 was significantly inhibited and its migration length (0.45 +/- 0.05) was significantly shorter than that before the transfection (0.81 +/- 0.11, chi(2) = 16.854, P < 0.05).

CONCLUSIONS

TGFbeta1 may contribute to EMT in esophageal squamous cell carcinoma. TGFbeta1-ASODN leads to an over-expression of E-cadherin and a down-regulation of vimentin, along with the morphological alterations and migration inhibition, indicating that a blockage of TGFbeta1 suppresses EMT in esophagus squamous cell carcinoma.

摘要

目的

研究转化生长因子β1（TGFβ1）在食管鳞状细胞癌上皮-间质转化（EMT）调控中的功能作用，以及TGFβ1反义寡脱氧核苷酸（TGFβ1-ASODN）阻断EMT的效果。

方法

用化学合成的TGFβ1-ASODN转染食管鳞状细胞癌细胞系EC9706。采用逆转录-聚合酶链反应（RT-PCR）、免疫组织化学和流式细胞术检测转染前后TGF-β1、E-钙黏蛋白和波形蛋白的蛋白及mRNA表达。记录形态学变化，并用划痕试验检测转染前后EC9706的迁移潜能。

结果

TGFβ1-ASODN转染后，EC9706中TGFβ1的mRNA（0.25±0.07）和蛋白（35.07%±1.42%）表达明显低于转染前（mRNA：0.43±0.09；蛋白：43.57%±1.77%，χ2 = 13.847，χ2 = 84.120，P < 0.05）。E-钙黏蛋白的mRNA（0.38±0.09）和蛋白（17.13%±1.45%）表达明显高于转染前（0.22±0.06；12.53%±1.31%，χ2 = 0.160，χ2 = 40.008，P < 0.05），波形蛋白的mRNA（0.73±0.07）和蛋白（14.15%±1.46%）表达明显低于转染前（0.89±0.09；17.97%±1.42%，χ2 = 0.160，χ2 = 21.103，P < 0.05）。划痕试验显示，转染后EC9706的迁移能力明显受到抑制，其迁移长度（0.45±0.05）明显短于转染前（0.81±0.11，χ2 = 16.854，P < 0.05）。