Liu Ming, Zeng Hui-chang, Zhang Xiao-li, Zhao Wen, Zhu Jing, Huang Jun-fu, Xia Mei
Department of Thoracic Surgery, Affiliated South-west Hospital, the Third Military Medical University, Chongqing 400038, China.
Zhonghua Wai Ke Za Zhi. 2008 Sep 1;46(17):1337-9.
To investigate the molecular alterations related to the carcinogenesis of esophageal squamous cell carcinoma (SCC), and also to find some molecular markers for the early detection of this cancer.
The resected tumor specimens and dysplasia tissues from 34 patients with esophageal cancer as well as their matched blood DNAs were analyzed for loss of heterozygosity (LOH) at 16 microsatellites by using PCR and fluorescence-based DNA sequencing technology. Mild and moderate dysplasia was classified as light-grade dysplasia (LGD), and severe dysplasia as high-grade dysplasia (HGD). The frequencies of LOH at 16 microsatellites were compared between tissue specimens with different histological diagnosis.
The total frequency of LOH for 16 microsatellites increased significantly in more severe lesions. There was significant difference in the frequency of LOH among LGD and HGD as well as SCC. A total of eight loci (D3S1597, D3S2452, D3S1285, D4S174, D5S2501, D9S125, D13S153 and D17S786) presented LOH in LGD samples. A reversion from LOH to retain of heterozygosity was observed at loci D3S2452, D4S174, D9S125 and D17S261 respectively when compared HGD with SCC samples obtained from 4 patients.
An accumulation of molecular alterations would be needed during the carcinogenesis of esophageal cancer. LOH analysis at some specific loci would be helpful for the early detection of esophageal cancer. The genetic heterogeneity possibly exists in the tumorigenesis of esophageal cancer.
研究与食管鳞状细胞癌（SCC）发生相关的分子改变，并寻找该癌症早期检测的分子标志物。
采用聚合酶链反应（PCR）和基于荧光的DNA测序技术，对34例食管癌患者的手术切除肿瘤标本、发育异常组织及其配对的血液DNA进行16个微卫星位点杂合性缺失（LOH）分析。轻度和中度发育异常归类为低级别发育异常（LGD），重度发育异常归类为高级别发育异常（HGD）。比较不同组织学诊断的组织标本中16个微卫星位点的LOH频率。
16个微卫星位点的LOH总频率在更严重的病变中显著增加。LGD、HGD和SCC之间的LOH频率存在显著差异。共有8个位点（D3S1597、D3S2452、D3S1285、D4S174、D5S2501、D9S125、D13S153和D17S786）在LGD样本中出现LOH。将4例患者的HGD样本与SCC样本比较时，分别在D3S2452、D4S174、D9S125和D17S261位点观察到从LOH到杂合性保留的逆转。
食管癌发生过程中需要分子改变的积累。某些特定位点的LOH分析有助于食管癌的早期检测。食管癌发生过程中可能存在基因异质性。
