Liu Ai-jun, Guan Zheng, Zhang Zhuo-mei, Wei Li-xin, Li Ya-li
Department of Pathology, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Zhonghua Bing Li Xue Za Zhi. 2008 Sep;37(9):584-8.
To investigate the distribution of ER isoforms in endometriosis and eutopic endometrium.
Tissue samples of patients with ovarian endometriosis, treated in People's Liberation Army General Hospital from January 2004 to December 2006, were retrieved. A total of 60 cases of ovarian endometriotic cysts with their corresponding eutopic endometrium (30 cases of proliferation phase and 30 of secretary phase eutopic endometrium) and 30 cases of normal endometrium (15 proliferative and 15 secretary phase endometrial samples respectively) were included. Expressions of ERalpha and ERbeta were analyzed using immunohistochemistry and the expression ratio was statistically analyzed by using SPSS 12.0 software.
Expressions of both ERalpha and ERbeta in epithelial cells were positively correlated with that of the stromal cells. The expression of ERalpha in eutopic endometrium (73.3% in epithelium and 76.7% in stroma) was significantly higher than that in ovarian endometriotic cysts (43.3% in epithelium and 46.7% in stroma), or normal control (56.7% in epithelium and 50.0% in stroma, respectively, each P < 0.05. However, the expression of ERbeta (90.0% in epithelium and 76.7% in stroma) was higher in ovarian endometriotic cysts than that in the eutopic endometrium (68.0% in epithelium and 63.3% in stroma respectively, P < 0.05), and ERbeta expression in eutopic endometrium was higher than that in the normal control endometrium (36.7% in epithelium and 26.7% in stroma, respectively, P < 0.05). The expressions of both ERalpha and ERbeta changed periodically in eutopic and normal endometrium, whereas ERalpha and ERbeta level were less variable in the ectopic endometrium. The expression of ERbeta was statistically higher than that of ERalpha (P < 0.05) in ectopic endometrium, whereas no significant difference was seen between the two isoforms in the eutopic or normal endometrium.
Both ERalpha and ERbeta have higher expression levels in eutopic endometrium of patients with ovarian endometriotic cysts. ERbeta is predominantly expressed in endometriotic cysts, where the expression of ERalpha is limited. The different distribution of ERalpha and ERbeta may play an important role in the development of ovarian endometriosis.
研究雌激素受体（ER）亚型在子宫内膜异位症及在位内膜中的分布情况。
收集2004年1月至2006年12月在解放军总医院接受治疗的卵巢子宫内膜异位症患者的组织样本。共纳入60例卵巢子宫内膜异位囊肿及其相应的在位内膜（增殖期在位内膜30例，分泌期在位内膜30例），以及30例正常子宫内膜（增殖期和分泌期内膜样本各15例）。采用免疫组织化学法分析ERα和ERβ的表达情况，并使用SPSS 12.0软件对表达率进行统计学分析。
上皮细胞和间质细胞中ERα和ERβ的表达均呈正相关。在位内膜中ERα的表达（上皮细胞中为73.3%，间质细胞中为76.7%）显著高于卵巢子宫内膜异位囊肿（上皮细胞中为43.3%，间质细胞中为46.7%）或正常对照（上皮细胞中分别为56.7%，间质细胞中为50.0%，各P<0.05）。然而，卵巢子宫内膜异位囊肿中ERβ的表达（上皮细胞中为90.0%，间质细胞中为76.7%）高于在位内膜（上皮细胞中分别为68.0%，间质细胞中为63.3%，P<0.05），且在位内膜中ERβ的表达高于正常对照子宫内膜（上皮细胞中分别为36.7%，间质细胞中为26.7%，P<0.05）。在位内膜和正常子宫内膜中ERα和ERβ的表达均呈周期性变化，而异位内膜中ERα和ERβ的水平变化较小。异位内膜中ERβ的表达在统计学上高于ERα（P<0.05），而在位内膜或正常子宫内膜中两种亚型之间无显著差异。
卵巢子宫内膜异位囊肿患者的在位内膜中ERα和ERβ均有较高的表达水平。ERβ主要表达于子宫内膜异位囊肿中，而ERα的表达受限。ERα和ERβ的不同分布可能在卵巢子宫内膜异位症的发生发展中起重要作用。
