Tam Hann W, Huang Yu-Chen, Tam Ming F
Institute of Molecular Biology, Academia Sinica, NanKang Taipei, Taiwan, ROC.
Anal Biochem. 2009 Mar 1;386(1):123-5. doi: 10.1016/j.ab.2008.11.028. Epub 2008 Nov 27.
We describe here the fabrication of a cost-effective semi-dry blotting apparatus for the transfer of proteins onto membranes. Graphite sheets were used as electrodes. Protein mixtures were separated on NuPAGE 4% to 12% polyacrylamide gradient gels. With a Tris-bicine buffer, we demonstrated that close to 80% of the proteins with apparent molecular mass of 80kDa or less were removed from the gels after 8min of blotting. The process is much faster than the techniques reported previously in the literature.
我们在此描述了一种用于将蛋白质转移到膜上的具有成本效益的半干印迹装置的制造方法。使用石墨片作为电极。蛋白质混合物在NuPAGE 4%至12%聚丙烯酰胺梯度凝胶上进行分离。使用Tris-双甘氨肽缓冲液，我们证明在印迹8分钟后，表观分子量为80kDa或更低的蛋白质中近80%从凝胶中被去除。该过程比文献中先前报道的技术要快得多。
