Dehareng D, Godeau J M
Department of Biochemistry, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
Arch Tierernahr. 1991 May;41(4):427-36. doi: 10.1080/17450399109428483.
The present work was an attempt to determine whether the variations in ruminal ammonia concentrations could be directly correlated to corresponding changes in total protozoa numbers of cattle. Four dry Friesian cows fed with hay-based rations were used through several experiments in which the twice daily feeding (6.15-15.30 h) as well as a 30 h-fasting period were studied. Simultaneously to a continuous or a regular collection of rumen liquor (for NH3), samples of rumen contents (for total protozoa numbers) were regularly withdrawn from the ventral sac. Any definite nycthemeral cycle of the protozoa numbers could not be related to feeding time. Moreover, no significant relationship could be found between the ruminal ammonia and the corresponding total protozoa numbers measured in the nocturnal interprandial period or during starvation. The data suggest that the nycthemeral ammonia profiles recorded in cattle are not directly related to protozoal activity.
本研究旨在确定瘤胃氨浓度的变化是否与牛总原生动物数量的相应变化直接相关。通过几个实验，使用了四头以干草为日粮的干奶期弗里生奶牛，研究了每日两次喂食（6:15 - 15:30）以及30小时禁食期。在连续或定期收集瘤胃液（用于测定氨）的同时，定期从腹囊抽取瘤胃内容物样本（用于测定总原生动物数量）。原生动物数量不存在任何明确的昼夜周期与喂食时间相关。此外，在夜间采食间期或饥饿期间测得的瘤胃氨与相应的总原生动物数量之间未发现显著关系。数据表明，牛记录的昼夜氨谱与原生动物活性没有直接关系。
