烷基链接物并入 siRNA 对 RNA 干扰的影响。
Effect of incorporation of alkyl linkers into siRNAs on RNA interference.
机构信息
Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
出版信息
Bioorg Med Chem Lett. 2009 Feb 1;19(3):875-7. doi: 10.1016/j.bmcl.2008.11.107. Epub 2008 Dec 6.
PMID:19095446
Abstract
Unintended (off-target) transcript silencing is a critical problem associated with RNA interference (RNAi)-based therapeutic applications. This paper shows that the incorporation of appropriate alkyl linkers at the center of the sense strands can suppress the off-target effects induced by the sense strands without reducing the RNAi-inducing activity of the antisense strands.
摘要
非预期（脱靶）转录沉默是与基于 RNA 干扰（RNAi）的治疗应用相关的一个关键问题。本文表明，在正义链的中心引入适当的烷基连接物可以抑制正义链诱导的脱靶效应，而不会降低反义链的 RNAi 诱导活性。
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