Saad Reda S, Lindner Jennifer L, Liu Yulin, Silverman Jan F
Department of Pathology, Allegheny General Hospital.
Am J Clin Pathol. 2009 Jan;131(1):92-8. doi: 10.1309/AJCPKWUQSIPVG90H.
We studied tumor lymphatic vascular density (LVD) as a predictive marker for the risk of lymph node (LN) metastasis and its relationship to other prognostic parameters and survival in 75 patients with esophageal adenocarcinoma. Samples were immunostained for D2-40, CD31, and vascular endothelial growth factor (VEGF). Microvessels were counted in densely vascular/lymphatic foci (hot spots) at x400 field (0.17 mm2). Intensity of staining for VEGF was scored on a 2-tiered scale. CD31 microvessel counts showed significant correlation with tumor stage and patient survival (P < .01). D2-40 LVD demonstrated a significant correlation with LN metastases, lymphovascular invasion, and tumor stage (r = 0.45, r = 0.47, and r = 0.37, respectively) and with shorter disease-free survival. D2-40 detected lymphovascular invasion in 29 of 75 cases, more than with CD31 (23/75) and H&E (18/75). VEGF was expressed in 48 (64%) of 75 cases and was significantly correlated with lymphovascular invasion, LN metastases, and overall survival. Our study showed that angiogenesis and lymphangiogenesis have important roles in the progression of esophageal adenocarcinoma.
我们研究了肿瘤淋巴管密度（LVD）作为预测食管癌患者发生淋巴结（LN）转移风险的标志物，及其与其他预后参数和生存率的关系，共纳入75例食管腺癌患者。样本进行了D2-40、CD31和血管内皮生长因子（VEGF）免疫染色。在400倍视野（0.17mm²）下，对血管/淋巴管密集区域（热点）的微血管进行计数。VEGF染色强度按两级评分。CD31微血管计数与肿瘤分期和患者生存率显著相关（P <.01）。D2-40 LVD与LN转移、淋巴管浸润和肿瘤分期显著相关（分别为r = 0.45、r = 0.47和r = 0.37），且与无病生存期较短相关。D2-40在75例中的29例检测到淋巴管浸润，多于CD31（23/75）和苏木精-伊红染色（H&E，18/75）。75例中有48例（64%）表达VEGF，且与淋巴管浸润、LN转移和总生存期显著相关。我们的研究表明，血管生成和淋巴管生成在食管腺癌进展中起重要作用。
