Forné Montserrat, Fernández-Bañares Fernando, González-Mínguez Clarisa, Casalots Jaume, Poblet-Mas Núria, Garcia-Gil L Jesús, Esteve Maria, Rosinach Mercè, Espinós Jorge, Loras Carme, Salas Antonio, Viver Josep M
Department of Gastroenterology, Hospital Universitari Mútua Terrassa, Terrassa, Spain.
Am J Clin Pathol. 2009 Jan;131(1):99-105. doi: 10.1309/AJCPNP7BK3KUUOJL.
Our aim was to evaluate the usefulness of the monoclonal antibody Das-1 as a premalignant marker of gastric intestinal metaplasia (GIM) associated with gastric cancer and its association with mucin expression. We evaluated Das-1 and mucin expression in 4 groups: 1 (n = 50), gastric carcinoma, paired samples of the cancer area and GIM away from the tumor; 2 (n = 25), gastric or duodenal ulcer with Helicobacter pylori infection with GIM and chronic gastritis; 3 (n = 25),H pylori- autoimmune chronic atrophic gastritis with GIM; and 4 (n = 25),H pylori- chronic gastritis without GIM. Das-1 immunostaining was observed in 20 (40%) of 50 cases in cancer areas. The expression of Das-1 in GIM from group 1 cases away from the cancer area was different from that in GIM from nontumor cases (groups 2 and 3): 13 (26%) of 50 vs 2 (8%) and 0 (0%) of 25 (P = .004). There was no association between Das-1 and mucin expression. Das-1 expression was associated with GIM from patients with gastric cancer. However, this relation was weaker than previously reported, precluding clinical usefulness as a premalignant marker of GIM.
我们的目的是评估单克隆抗体Das-1作为与胃癌相关的胃肠化生（GIM）的癌前标志物的效用及其与黏蛋白表达的关联。我们评估了4组中Das-1和黏蛋白的表达情况：1组（n = 50），胃癌，癌区及远离肿瘤的GIM配对样本；2组（n = 25），伴有GIM和慢性胃炎的幽门螺杆菌感染的胃溃疡或十二指肠溃疡；3组（n = 25），伴有GIM的幽门螺杆菌相关性自身免疫性慢性萎缩性胃炎；4组（n = 25），无GIM的幽门螺杆菌相关性慢性胃炎。在癌区的50例病例中有20例（40%）观察到Das-1免疫染色。1组远离癌区的GIM中Das-1的表达与非肿瘤病例（2组和3组）的GIM中Das-1的表达不同：50例中的13例（26%）对比25例中的2例（8%）和0例（0%）（P = .004）。Das-1与黏蛋白表达之间无关联。Das-1表达与胃癌患者的GIM相关。然而，这种关系比之前报道的要弱，排除了其作为GIM癌前标志物的临床效用。
