da Silva Benedito Borges, Lopes-Costa Pedro Vitor, dos Santos Alesse Ribeiro, Pires Cleicilene Gomes, Borges Carine Soares, Gontijo José Antônio
Department of Gynecology, Federal University of Piauí, Teresina, Piauí, Brazil.
Hum Reprod. 2009 Mar;24(3):705-9. doi: 10.1093/humrep/den451. Epub 2008 Dec 17.
Hyperandrogenism and chronic anovulation are basic characteristics of polycystic ovary syndrome (PCOS), and androgens from the adrenal glands play an important role in the hyperandrogenism. Our aim was to evaluate the proliferative activity in the zona reticularis cells of the adrenal cortex of female rats in persistent estrus, a model developed to mimic PCOS.
Forty-four female Wistar-Hannover rats were randomly divided into two groups: control (n = 17) and animals which received 1.25 mg testosterone propionate s.c. on the second day of life (n = 27). At 90 days of age, after confirmation of persistent estrus, the animals were sacrificed, and the adrenal glands were removed and fixed in 10% buffered formalin to investigate Ki-67 antigen (marker of proliferation) expression by immunohistochemical analysis. Student's t-test and Levene's test were used in the statistical analysis.
The mean percentage of Ki-67-stained nuclei per 1000 cells in the zona reticularis of the adrenal cortex was 15.58 +/- 1.14 (SEM) and 51.59 +/- 1.81 in the control and persistent estrus animals, respectively (P < 0.001).
Proliferative activity in the zona reticularis cells of the adrenal cortex of the androgenized female rats was significantly greater than that of the control animals.
高雄激素血症和慢性无排卵是多囊卵巢综合征（PCOS）的基本特征，肾上腺来源的雄激素在高雄激素血症中起重要作用。我们的目的是评估持续发情期雌性大鼠肾上腺皮质网状带细胞的增殖活性，该模型用于模拟PCOS。
44只雌性Wistar-Hannover大鼠随机分为两组：对照组（n = 17）和在出生后第二天皮下注射1.25 mg丙酸睾酮的动物组（n = 27）。90日龄时，在确认处于持续发情期后，处死动物，取出肾上腺并固定于10%缓冲福尔马林中，通过免疫组织化学分析研究Ki-67抗原（增殖标志物）的表达。统计分析采用Student's t检验和Levene检验。
肾上腺皮质网状带中每1000个细胞中Ki-67染色细胞核的平均百分比在对照组和持续发情期动物中分别为15.58±1.14（SEM）和51.59±1.81（P < 0.001）。
雄激素化雌性大鼠肾上腺皮质网状带细胞的增殖活性显著高于对照动物。
