Bruch R C, Carr V M
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208.
Brain Res. 1991 May 31;550(1):133-6. doi: 10.1016/0006-8993(91)90416-s.
Neurofilament expression in peripheral olfactory neurons of adult rats was investigated by immunoblotting and immunohistochemistry using monoclonal antibodies specific for each of the 3 neurofilament proteins. Immunoblotting analysis of olfactory epithelium extracts demonstrated the presence of only the 200 kDa (NFH) polypeptide; the 68 kDa (NFL) and 160 kDa (NFM) neurofilaments were not detected. Similarly, no immunoreactivity was observed in tissue sections using the NFL and NFM antibodies. In contrast, when sections were probed with the antibody to NFH, immunoreactivity was localized primarily in the dendritic knobs and near the cell bodies of the receptor cells.
利用针对3种神经丝蛋白各自的单克隆抗体，通过免疫印迹法和免疫组织化学法研究成年大鼠外周嗅觉神经元中的神经丝表达。对嗅觉上皮提取物的免疫印迹分析表明仅存在200 kDa（NFH）多肽；未检测到68 kDa（NFL）和160 kDa（NFM）神经丝。同样，使用NFL和NFM抗体在组织切片中未观察到免疫反应性。相比之下，当用抗NFH抗体探测切片时，免疫反应性主要定位于受体细胞的树突小结和细胞体附近。
