Würzner R, Schuff-Werner P, Franzke A, Nitze R, Oppermann M, Armstrong V W, Eisenhauer T, Seidel D, Götze O
Department of Immunology, Georg-August-University, Göttingen, Germany.
Eur J Clin Invest. 1991 Jun;21(3):288-94. doi: 10.1111/j.1365-2362.1991.tb01372.x.
The heparin-induced extracorporeal elimination of low density lipoproteins (LDL) is a well-established clinical procedure to markedly reduce cholesterol levels. The biocompatibility of this artificial filter system (HELP) was investigated by quantitation of representative complement proteins within the extracorporeal circuit using established ELISA procedures, based on monoclonal antibodies recognizing exclusively either native (C6, C7) or activated proteins (act.C3, C5a, TCC). HELP was found to be a self-limiting extracorporeal system with respect to complement activation, since act.C3 and TCC, generated mainly at the plasma filter, were partially adsorbed to the following HELP specific filters to concentrations which were lower than those obtained before the plasma filter. C5a, which increased 14.5-fold at the plasma filter was not eliminated by the following filters; however, elevated levels were not found in the patients at the end of apheresis and no leucocytopenia was observed.
肝素诱导的体外低密度脂蛋白（LDL）清除是一种成熟的临床操作，可显著降低胆固醇水平。基于识别天然蛋白（C6、C7）或活化蛋白（活化C3、C5a、末端补体复合物）的单克隆抗体，采用既定的ELISA程序，通过定量体外循环中的代表性补体蛋白，研究了这种人工滤器系统（HELP）的生物相容性。结果发现，就补体激活而言，HELP是一个自我限制的体外系统，因为主要在血浆滤器处产生的活化C3和末端补体复合物，部分吸附到后续的HELP特异性滤器上，其浓度低于血浆滤器前的浓度。在血浆滤器处增加了14.5倍的C5a未被后续滤器清除；然而，在单采结束时患者体内未发现其水平升高，也未观察到白细胞减少。
