Liu Qing-Guo, Ji Bo, Qin Wei-Lan, Fu Yong-Jun, Qin Yi, Zeng Gui-Gang, Ren Xiao-Xuan
School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhen Ci Yan Jiu. 2008 Oct;33(5):306-9.
To observe the effect of acupotomy lysis on serum matrix metalloproteinase (MMP)-3 and MMP-13 contents in osteoarthritis (OA) rabbits.
A total of 34 New Zealand rabbits were randomly divided into normal group (n = 7), model group (n = 7), acupotomy group (n = 10) and electroacupuncture (EA) group (n = 10). OA model was established by left-hindlimb-joint-immobilization method. Acupotomy lysis was applied to the affected joint, once a week for 3 weeks. EA (2/100 Hz, 2 mA) was applied to "Yinlingquan" (SP 9), "Yanglingquan" (GB 34) and "Liangqiu" (ST 34) on the left hindlimb for 10 min, 3 times a week for 3 weeks. Serum MMP-3 and MMP-13 contents were detected with enzyme linked immunosorbent assay (ELISA) method.
In comparison with model group (grade III: 1 case; grade: 4 cases), the left knee-joint-motion range of rabbits in acupotomy and EA groups (grade I: 1 and 1 case; grade II: 4 and 3 cases; grade III: 2 and 2 cases; grade IV: 1 and 2 cases separately) increased significantly (P < 0.05). Compared with normal group, serum MMP-3 and MMP-13 contents in model group increased significantly (P < 0.05); while compared with model group, both MMP-3 and MMP-13 levels of acupotomy and EA groups reduced considerably (P < 0.05). No obvious difference was found between acupotomy and EA groups in the left knee-joint motion range, and serum MMP-3 and MMP-13 levels (P > 0.05).
Acupotomy is effective in lowering serum MMP-3 and MMP-13 levels, and in improving OA rabbits' knee-joint motion range.
观察针刀松解术对骨关节炎（OA）兔血清基质金属蛋白酶（MMP）-3和MMP-13含量的影响。
将34只新西兰兔随机分为正常组（n = 7）、模型组（n = 7）、针刀组（n = 10）和电针组（n = 10）。采用左后肢关节制动法建立OA模型。对患侧关节进行针刀松解，每周1次，共3周。将电针（2/100Hz，2mA）施加于左后肢的“阴陵泉”（SP 9）、“阳陵泉”（GB 34）和“梁丘”（ST 34），每次10分钟，每周3次，共3周。采用酶联免疫吸附测定（ELISA）法检测血清MMP-3和MMP-13含量。
与模型组（Ⅲ级：1例；Ⅳ级：4例）相比，针刀组和电针组兔左膝关节活动范围（Ⅰ级：各1例；Ⅱ级：各4例和3例；Ⅲ级：各2例；Ⅳ级：分别为1例和2例）明显增加（P < 0.05）。与正常组相比，模型组血清MMP-3和MMP-13含量明显增加（P < 0.05）；而与模型组相比，针刀组和电针组的MMP-3和MMP-13水平均显著降低（P < 0.05）。针刀组和电针组在左膝关节活动范围以及血清MMP-3和MMP-13水平方面无明显差异（P > 0.05）。
针刀术能有效降低血清MMP-3和MMP-13水平，并改善OA兔的膝关节活动范围。
