鉴定和功能分析Alternaria brassicicola 中 brassicicene C 生物合成基因簇。
Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola.
机构信息
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
出版信息
Bioorg Med Chem Lett. 2009 Feb 1;19(3):870-4. doi: 10.1016/j.bmcl.2008.11.108. Epub 2008 Dec 6.
The biosynthetic gene cluster of brassicicene C was identified in Alternaria brassicicola strain ATCC 96836 from genome database search. In vivo and in vitro study clearly revealed the function of Orf8 and Orf6 as a fusicoccadiene synthase and methyltransferase, respectively. The understanding toward the biosynthetic pathway promises construction of this type of diterpene compounds with genetic engineering.
从基因组数据库搜索中鉴定出 Alternaria brassicicola 菌株 ATCC 96836 中 brassicicene C 的生物合成基因簇。体内和体外研究清楚地揭示了 Orf8 和 Orf6 作为 fusicoccadiene 合酶和甲基转移酶的功能。对生物合成途径的理解有望通过基因工程构建此类二萜化合物。
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