[急性冠状动脉综合征患者CD4+CD28阴性T淋巴细胞上电压门控钾通道Kv1.3上调]

[Upregulated voltage-gated potassium channel Kv1.3 on the CD4+CD28null T lymphocyte from patients with acute coronary syndrome].

作者信息

Huang Shen, Tang Jia-rong, Zhang Cun-tai, Sun Li-ping, Guo Li-fen, Sun Meng

机构信息

Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Xin Xue Guan Bing Za Zhi. 2008 Jul;36(7):602-7.

PMID:19100087

Abstract

OBJECTIVE

The purpose of our study is to observe the voltage-gated potassium channel Kv1.3 expression on CD4+CD28null T cells from the peripheral blood of ACS patients by the patch clamp technique.

METHODS

Kv1.3 potassium channels expression from 17 patients with ACS and 11 healthy age-matched normal controls was detected in single cell (CD4+CD28null T cells and CD4+CD28+ T cells) by fluorescence microscopy and patch clamp.

RESULTS

The percent of CD4+CD28nullT cells are higher in the ACS (6.97% +/- 2.05%) than that in the controls (1.38% +/- 0.84%, P < 0.05). The concentration of hsCRP is directly correlated with the number of the CD4+CD28null T cells in the ACS (r = 0.52, P < 0.05). The conductance [(6.89 +/- 1.17) nS vs. (3.36 +/- 0.66) nS], dens [(1.95 +/- 0.80) n/microm2 vs. (1.13 +/- 0.57) n/microm2] and numbers [(574.5 +/- 97.6) n/cell vs. (280.3 +/- 55.3) n/cell] of the Kv1.3 channels on the CD4+CD28null T cells are significantly higher than those on the CD4+CD28+ T cells (all P < 0.01) in ACS patients, but were similar on CD4+CD28+ T cells between ACS patients and controls.

CONCLUSION

The CD4+CD28null T cells in the ACS and the numbers of Kv1.3 channels on the CD4+CD28null T cells from the ACS patients are significantly upregulated and might contribute to the pathogenesis of ACS.

摘要

目的

本研究旨在通过膜片钳技术观察急性冠状动脉综合征（ACS）患者外周血中CD4+CD28null T细胞上电压门控钾通道Kv1.3的表达情况。

方法

采用荧光显微镜和膜片钳技术，在单细胞水平（CD4+CD28null T细胞和CD4+CD28+ T细胞）检测17例ACS患者及11例年龄匹配的健康正常对照者的Kv1.3钾通道表达。

结果

ACS患者中CD4+CD28null T细胞的百分比（6.97%±2.05%）高于对照组（1.38%±0.84%，P<0.05）。ACS患者中高敏C反应蛋白（hsCRP）浓度与CD4+CD28null T细胞数量直接相关（r=0.52，P<0.05）。ACS患者中，CD4+CD28null T细胞上Kv1.3通道的电导[（6.89±1.17）nS对（3.36±0.66）nS]、密度[（1.95±0.80）n/μm2对（1.13±0.57）n/μm2]和数量[（574.5±97.6）n/细胞对（280.3±55.3）n/细胞]均显著高于CD4+CD28+ T细胞（均P<0.01），但ACS患者与对照组的CD4+CD28+ T细胞上的这些指标相似。