Department of Internal Disease, Geriatrics and Allergology, Medical University, Wrocław, Poland.
J Membr Biol. 2011 Jul;242(1):23-9. doi: 10.1007/s00232-011-9373-7. Epub 2011 Jun 25.
Kv1.3 channels play an important role in T lymphocytes function. CD4(+) and CD4(+)CD25(+) T cells are two broad categories of T cells that are critically involved in the immunoresponse to allergens and that are also a major target for allergen immunotherapy. The aim of the study was to evaluate the effects of venom immunotherapy (VIT) on the activity of Kv1.3. channels on noncultured subsets: CD4(+) and CD4(+)CD25(+) T cells of insect venom allergic patients. Eleven patients with allergic reactions to bee or wasp venoms participated in the study. The patients were provided VIT according to the ultrarush protocol. CD4(+) and CD4(+)CD25(+) T cells were isolated from peripheral blood mononuclear cells of VIT-treated patients by an immunomagnetic method. We used the whole-cell patch clamp technique to investigate the whole potassium chord conductance (gK) of Kv1.3. channels in CD4(+) and CD4(+)CD25(+) T cells of venom-sensitive patients before and during the course of VIT. The conductance of Kv1.3. channels on CD4(+)CD25(+) T cells decreased during the course of VIT. On day 0 it was 0.054 ± 0.07 [nS], and on day 70 it was 0.008 ± 0.09 [nS] (P = 0.03). The observed decrease of the gK of the Kv1.3 channels in the subpopulation of activated T cells may contribute to T cell tolerance and functional unresponsiveness of these cells to allergen in the early stages of VIT.
Kv1.3 通道在 T 淋巴细胞功能中发挥重要作用。CD4(+)和 CD4(+)CD25(+)T 细胞是两类广泛的 T 细胞，它们在免疫反应中至关重要，也是过敏原免疫治疗的主要目标。本研究旨在评估毒液免疫疗法（VIT）对非培养亚群：昆虫毒液过敏患者的 CD4(+)和 CD4(+)CD25(+)T 细胞中 Kv1.3 通道活性的影响。11 名对蜂或黄蜂毒液过敏的患者参与了这项研究。根据超速方案为患者提供 VIT。通过免疫磁珠方法从 VIT 治疗患者的外周血单个核细胞中分离 CD4(+)和 CD4(+)CD25(+)T 细胞。我们使用全细胞膜片钳技术研究了毒液敏感患者的 CD4(+)和 CD4(+)CD25(+)T 细胞中 Kv1.3 通道的全钾通道电导（gK），在 VIT 之前和期间。CD4(+)CD25(+)T 细胞上 Kv1.3 通道的电导在 VIT 期间降低。在第 0 天为 0.054±0.07[nS]，在第 70 天为 0.008±0.09[nS]（P=0.03）。在 VIT 的早期阶段，激活 T 细胞亚群中 Kv1.3 通道 gK 的观察到的降低可能有助于 T 细胞耐受和这些细胞对过敏原的功能无反应性。
