Jiao Y, Peng Z-H, Zhang J-Y, Qin J, Zhong C-P
Shanghai Organ Transplantation Clinical Medical Center, the First People's Hospital Affiliated Shanghai Jiao Tong University, Shanghai, China.
Transplant Proc. 2008 Dec;40(10):3615-8. doi: 10.1016/j.transproceed.2008.04.026.
An in vivo method for islet visualization using magnetic resonance imaging (MRI), using superparamagnetic iron oxide (SPIO), has been described. Herein we have developed a protocol that uses cationic liposomes to increase the efficiency of islet cell labeling by SPIO in vitro. Fresh islet cells were incubated in RPMI-1640 medium, to which had been added a range of concentrations of ferucarbotran, a SPIO contrast agent. At each SPIO concentration, duplicate samples were incubated with versus without addition of cationic liposomes. We measured intracellular iron concentration, cell viability, and insulin-release function after labeling. We observed that the amount of iron bound to islet cells increased as the concentration of added SPIO increased. The presence of liposomes increased labeling efficiency at each SPIO concentration. In vitro MRI confirmed the effect of liposomes to improve labeling efficiency of islet cells by SPIO.
已经描述了一种使用磁共振成像（MRI）和超顺磁性氧化铁（SPIO）进行胰岛可视化的体内方法。在此，我们开发了一种方案，该方案使用阳离子脂质体在体外提高SPIO对胰岛细胞的标记效率。将新鲜的胰岛细胞在RPMI-1640培养基中孵育，该培养基中添加了一系列浓度的SPIO造影剂 ferucarbotran。在每个SPIO浓度下，将重复样本分别在添加和不添加阳离子脂质体的情况下进行孵育。我们在标记后测量了细胞内铁浓度、细胞活力和胰岛素释放功能。我们观察到，与胰岛细胞结合的铁量随着添加的SPIO浓度的增加而增加。在每个SPIO浓度下，脂质体的存在都提高了标记效率。体外MRI证实了脂质体对提高SPIO对胰岛细胞标记效率的作用。
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