Endocrinology and Metabolic Diseases, University Medical Center, Johannes Gutenberg University Mainz, Germany.
Xenotransplantation. 2013 Jul-Aug;20(4):219-26. doi: 10.1111/xen.12042. Epub 2013 Jun 23.
Islet cell transplantation is a promising option for the restoration of normal glucose homeostasis in patients with type 1 diabetes. Because graft volume is a crucial issue in islet transplantations for patients with diabetes, we evaluated a new method for increasing functional tissue yield in xenogeneic grafts of encapsulated islets. Islets were labeled with three different superparamagnetic iron oxide nano particles (SPIONs; dextran-coated SPION, siloxane-coated SPION, and heparin-coated SPION). Magnetic separation was performed to separate encapsulated islets from the empty capsules, and cell viability and function were tested. Islets labeled with 1000 μg Fe/ml dextran-coated SPIONs experienced a 69.9% reduction in graft volume, with a 33.2% loss of islet-containing capsules. Islets labeled with 100 μg Fe/ml heparin-coated SPIONs showed a 46.4% reduction in graft volume, with a 4.5% loss of capsules containing islets. No purification could be achieved using siloxane-coated SPIONs due to its toxicity to the primary islets. SPION labeling of islets is useful for transplant purification during islet separation as well as in vivo imaging after transplantation. Furthermore, purification of encapsulated islets can also reduce the volume of the encapsulated islets without impairing their function by removing empty capsules.
胰岛细胞移植是恢复 1 型糖尿病患者正常血糖稳态的一种很有前途的选择。由于胰岛移植中移植物体积是糖尿病患者的一个关键问题，我们评估了一种新的方法来增加包封胰岛异种移植物中功能性组织的产量。将胰岛用三种不同的超顺磁性氧化铁纳米颗粒（SPION；葡聚糖包被的 SPION、硅氧烷包被的 SPION 和肝素包被的 SPION）进行标记。通过磁分离从空胶囊中分离包封的胰岛，并测试细胞活力和功能。用 1000μg Fe/ml 葡聚糖包被的 SPION 标记的胰岛的移植物体积减少了 69.9%，含胰岛的胶囊损失了 33.2%。用 100μg Fe/ml 肝素包被的 SPION 标记的胰岛的移植物体积减少了 46.4%，含胰岛的胶囊损失了 4.5%。由于硅氧烷包被的 SPION 对原代胰岛具有毒性，因此无法进行纯化。胰岛的 SPION 标记可用于胰岛分离过程中的移植纯化以及移植后的体内成像。此外，通过去除空胶囊，包裹胰岛的纯化还可以在不损害其功能的情况下减少包裹胰岛的体积。
