Sosnowski J T, DeHaven J I, Riggs D R, Lamm D L
Department of Urology, School of Medicine, West Virginia University, Morgantown 26506.
J Urol. 1991 Oct;146(4):1164-7. doi: 10.1016/s0022-5347(17)38032-1.
The antitumor effect of intralesionally administered recombinant interleukin-2, alone or in combination with recombinant interferon gamma was studied in murine transitional cell carcinoma, MBT2. In the initial prophylactic model treatment was started at day one at the site of tumor inoculation. Maximal and significant reduction in tumor volume occurred in groups receiving 4,000 units of recombinant interleukin 2 and 10(7) colony forming units Bacillus Calmette Guerin (p less than 0.00001 vs saline control). In the same experiment, a reduction in tumor incidence and increase in survival occurred in groups receiving 4,000 units of recombinant interleukin 2, 1,000 units of recombinant interleukin 2 plus 2,000 units of recombinant interferon gamma, as well as 10(7) colony forming units Bacillus Calmette Guerin relative to saline control (p less than 0.005). The dose-response effect of recombinant interleukin 2 alone was also tested in a model of an established transitional cell carcinoma. Intralesional injection treatments were initiated after tumors were palpable. Reduction in tumor volume was observed in the group receiving 8,000 units of recombinant interleukin 2 (p = 0.01 vs saline control), but no significant advantage in survival was noted.
在小鼠移行细胞癌MBT2中研究了瘤内注射重组白细胞介素-2单独或与重组干扰素γ联合使用的抗肿瘤作用。在初始预防模型中，于肿瘤接种部位在第1天开始治疗。接受4000单位重组白细胞介素-2和10⁷菌落形成单位卡介苗的组中，肿瘤体积出现最大且显著的减小（与生理盐水对照组相比，p＜0.00001）。在同一实验中，相对于生理盐水对照组，接受4000单位重组白细胞介素-2、1000单位重组白细胞介素-2加2000单位重组干扰素γ以及10⁷菌落形成单位卡介苗的组中，肿瘤发生率降低且生存率提高（p＜0.005）。还在已建立的移行细胞癌模型中测试了重组白细胞介素-2单独的剂量反应效应。在肿瘤可触及后开始瘤内注射治疗。接受8000单位重组白细胞介素-2的组中观察到肿瘤体积减小（与生理盐水对照组相比，p = 0.01），但未观察到生存方面的显著优势。
