Maurais Emilie, Cantin Réjean, Tremblay Michel J
Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval, Canada.
Virology. 2009 Mar 1;385(1):227-32. doi: 10.1016/j.virol.2008.11.033. Epub 2008 Dec 19.
CD40 ligand (CD40L) is mainly expressed in activated CD4(+)T cells and interacts with CD40 on antigen-presenting cells to regulate both humoral and cellular immune responses. We previously reported that CD40L is acquired by emerging HIV-1 particles. Here we demonstrate that both wild-type and a non-functional mutated form of CD40L are incorporated within HIV-1. Importantly, we show that wild-type CD40L remains functional since CD40L-bearing virions mediate NF-kappaB activation in a CD40-expressing reporter cell line and induce secretion of the chemokine IL-8 by monocyte-derived macrophages. These results suggest a possible means exploited by HIV-1 to attract susceptible target cells to the site of infection, a process that might promote viral dissemination.
CD40配体（CD40L）主要表达于活化的CD4(+)T细胞中，并与抗原呈递细胞上的CD40相互作用，以调节体液免疫和细胞免疫反应。我们之前报道过，新出现的HIV-1颗粒会获得CD40L。在此我们证明，野生型和无功能的突变形式的CD40L均被整合到HIV-1中。重要的是，我们发现野生型CD40L仍具有功能，因为携带CD40L的病毒粒子在表达CD40的报告细胞系中介导NF-κB激活，并诱导单核细胞衍生的巨噬细胞分泌趋化因子IL-8。这些结果提示了HIV-1利用的一种可能方式，即将易感靶细胞吸引到感染部位，这一过程可能会促进病毒传播。
