Legagneux K, Bernard-Franchi G, Poncet F, La Roche A, Colard C, Fellmann D, Pralong F, Risold P Y
EA3922, Université de Franche-Comté, Faculté de Médecine et de Pharmacie, 19 rue Ambroise Paré, 25000 Besançon, France.
Neuropeptides. 2009 Feb;43(1):13-9. doi: 10.1016/j.npep.2008.11.001. Epub 2008 Dec 19.
Prepro-RFRP-containing neurons have recently been described in the mammalian brain. These neurons are only found in the tuberal hypothalamus. In this work, we have provided a detailed analysis of the distribution of cells expressing the RFRP mRNA, and found them in seven anatomical structures of the tuberal hypothalamus. No co-expression with melanin-concentrating hormone (MCH) or hypocretin (Hcrt), that are also described in neurons of the tuberal hypothalamus, was observed. Using the BrdU method, we found that all RFRP cell bodies are generated between E13 and E14. Thus, RFRP neurons form a specific cell population with a complex distribution pattern in the tuberal hypothalamus. However, they are generated in one peak. These observations are discussed with data concerning the distribution and genesis of the MCH and Hcrt cell populations that are also distributed in the tuberal hypothalamus.
最近在哺乳动物大脑中发现了含前体蛋白-促性腺激素释放激素相关肽（Prepro-RFRP）的神经元。这些神经元仅在下丘脑结节部被发现。在这项研究中，我们对表达RFRP mRNA的细胞分布进行了详细分析，并在丘脑下部的七个解剖结构中发现了它们。未观察到与同样在下丘脑结节部神经元中被描述的促黑素（MCH）或下丘脑分泌素（Hcrt）共表达。使用BrdU方法，我们发现所有RFRP细胞体在胚胎第13天到第14天之间产生。因此，RFRP神经元在下丘脑结节部形成了一个具有复杂分布模式的特定细胞群。然而，它们在一个峰值期产生。我们将结合同样分布在下丘脑结节部的MCH和Hcrt细胞群的分布及起源数据对这些观察结果进行讨论。
