Bergh C, Olsson J H, Hillensjö T
Department of Obstetrics and Gynecology, University of Göteborg, Sahlgrenska Hospital, Sweden.
Acta Endocrinol (Copenh). 1991 Aug;125(2):177-85. doi: 10.1530/acta.0.1250177.
The effect of IGF-I on steroidogenesis in human granulosa cells was studied. Granulosa cells were obtained from follicles of both natural and stimulated cycles. The cells were cultured 4 to 6 days and the effect of IGF-I (1 to 100 micrograms/l) on basal, LH- and FSH-stimulated steroidogenesis was studied. It was found that in granulosa cells from follicles of natural cycles, FSH as well as IGF-I significantly stimulated progesterone and estradiol production in a majority of the experiments. A synergistic effect of FSH and IGF-I could be seen when low (1 and 10 micrograms/l) concentrations of the two hormones were used. Also in granulosa luteal cells from stimulated cycles a stimulatory effect of IGF-I on estradiol as well as progesterone production was observed. The present results suggest that IGF-I in combination with gonadotropins has a physiological role in the human follicle in controlling differentiation of the granulosa cells.
研究了胰岛素样生长因子-I（IGF-I）对人颗粒细胞类固醇生成的影响。颗粒细胞取自自然周期和促排卵周期的卵泡。细胞培养4至6天，研究了IGF-I（1至100微克/升）对基础、促黄体生成素（LH）和促卵泡生成素（FSH）刺激的类固醇生成的影响。结果发现，在自然周期卵泡的颗粒细胞中，在大多数实验中，FSH以及IGF-I均显著刺激孕酮和雌二醇的生成。当使用低浓度（1和10微克/升）的这两种激素时，可观察到FSH和IGF-I的协同作用。在促排卵周期的颗粒黄体细胞中也观察到IGF-I对雌二醇以及孕酮生成的刺激作用。目前的结果表明，IGF-I与促性腺激素联合在人类卵泡中对颗粒细胞分化的控制具有生理作用。
