[小鼠肝脏二胺氧化酶对腐胺、亚精胺和精胺的氧化作用]
[Oxidation of putrescine, spermidine and spermine by diamino-oxidase from mouse liver].
作者信息
Siatkin S P, Galaev Iu V
出版信息
Biokhimiia. 1977 Jun;42(6):1010-3.
Optimal conditions to determine the activity of diaminooxidase in mouse liver homogenate are described. Maximal oxidation rate for putrescine was found to take place at a concentration of 20 mM and pH 9.5, and for spermidine and spermine--at 10 mM concentration and pH 9.2. The rate of tyramine oxidation was maximal at pH 7.8. Apparent KM values were 4.98-10(-3 M, 1-10(-3) M and 0.8-10(-3) M for putrescine, spermidine and spermine respectively. Hydroxylamine did not inhibit the rate of putrescin oxidation at optimal pH value.
本文描述了测定小鼠肝脏匀浆中二胺氧化酶活性的最佳条件。发现腐胺的最大氧化速率发生在浓度为20 mM和pH 9.5时，亚精胺和精胺的最大氧化速率发生在浓度为10 mM和pH 9.2时。酪胺氧化速率在pH 7.8时最大。腐胺、亚精胺和精胺的表观米氏常数分别为4.98×10⁻³ M、1×10⁻³ M和0.8×10⁻³ M。在最佳pH值下，羟胺不抑制腐胺的氧化速率。
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