一种对猫上皮嗜性病毒敏感的连续猫乳腺上皮细胞系的特性分析。
Characterization of a continuous feline mammary epithelial cell line susceptible to feline epitheliotropic viruses.
作者信息
Pesavento Patricia, Liu Hongwei, Ossiboff Robert J, Stucker Karla M, Heymer Anna, Millon Lee, Wood Jason, van der List Deborah, Parker John S L
机构信息
Department of Pathology, Microbiology and Immunology, University of California Davis, Davis, CA 95616, USA.
出版信息
J Virol Methods. 2009 Apr;157(1):105-10. doi: 10.1016/j.jviromet.2008.11.018. Epub 2009 Jan 14.
Mucosal epithelial cells are the primary targets for many common viral pathogens of cats. Viral infection of epithelia can damage or disrupt the epithelial barrier that protects underlying tissues. In vitro cell culture systems are an effective means to study how viruses infect and disrupt epithelial barriers, however no true continuous or immortalized feline epithelial cell culture lines are available. A continuous cell culture of feline mammary epithelial cells (FMEC UCD-04-2) that forms tight junctions with high transepithelial electrical resistance (>2000Omegacm(-1)) 3-4 days after reaching confluence was characterized. In addition, it was shown that FMECs are susceptible to infection with feline calicivirus (FCV), feline herpesvirus (FHV-1), feline coronavirus (FeCoV), and feline panleukopenia virus (FPV). These cells will be useful for studies of feline viral disease and for in vitro studies of feline epithelia.
黏膜上皮细胞是猫许多常见病毒病原体的主要靶标。上皮细胞的病毒感染会损害或破坏保护下层组织的上皮屏障。体外细胞培养系统是研究病毒如何感染和破坏上皮屏障的有效手段，然而目前尚无真正连续或永生化的猫上皮细胞系。对一种猫乳腺上皮细胞（FMEC UCD - 04 - 2）的连续细胞培养进行了特性描述，该细胞系在汇合后3 - 4天形成紧密连接，具有高跨上皮电阻（>2000Ωcm(-1)）。此外，研究表明FMECs对猫杯状病毒（FCV）、猫疱疹病毒（FHV - 1）、猫冠状病毒（FeCoV）和猫泛白细胞减少症病毒（FPV）敏感。这些细胞将有助于猫病毒性疾病的研究以及猫上皮细胞的体外研究。
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