Nilsson E, Ostwald K, Karlsson J O
Institute of Neurobiology, University of Göteborg, Sweden.
Mol Chem Neuropathol. 1991 Apr;14(2):99-111. doi: 10.1007/BF03159930.
Calpains and calpastatin in the brain of the rabbit were examined in experimental situations that could mimic some features of brain ischemia. Incubations of bisected brains in saline at 39 degrees C for 0.5, 1, or 1.5 h resulted in a decreased calpain I activity in the cytosol and in an increased hydrophobicity of cytosolic calpain II activity. Incubation of brain homogenates at different pH levels demonstrated an almost-complete transfer of calpains from the cytoplasmic compartment to the membranes when pH was lowered from 6 to 5. At pH values lower than 5, the total calpain activity (soluble plus membrane-bound) markedly decreased. No significant changes of calpastatin activity or its subcellular distribution was found following incubation of the homogenates at different pH levels.
在能够模拟脑缺血某些特征的实验条件下，对兔脑中的钙蛋白酶和钙蛋白酶抑制蛋白进行了检测。将切成两半的脑在39摄氏度的盐水中孵育0.5、1或1.5小时，导致胞质溶胶中钙蛋白酶I活性降低，且胞质钙蛋白酶II活性的疏水性增加。在不同pH水平下对脑匀浆进行孵育表明，当pH从6降至5时，钙蛋白酶几乎完全从细胞质区室转移到膜上。在pH值低于5时，总钙蛋白酶活性（可溶性加膜结合）显著降低。在不同pH水平下对匀浆进行孵育后，未发现钙蛋白酶抑制蛋白活性或其亚细胞分布有显著变化。
