van Gulik T M, Boudjema K, Platz K, Hullett D A, Starling J R, Southard J H, Sollinger H W, Belzer F O
Department of Surgery, University of Amsterdam, The Netherlands.
Transpl Int. 1991 Jun;4(2):110-5. doi: 10.1007/BF00336408.
Canine thyroid tissue (CTy) was subjected to hyperbaric oxygen culture (HOC) under conditions that affect immunoalteration in murine thyroid tissue (MTy). Survival of autografts and allografts implanted under the kidney capsule was determined after 21 days by 125I uptake and histology. Unlike MTy, autograft CTy subjected to normothermic HOC (95% O2, 5% CO2; 1.76 kg/cm2) for 48 h did not survive (0/8) whereas decrease of culture duration to 24 h resulted in autograft CTy survival (3/3). Under hypothermia (5 degrees C), HOC could be extended to 7 days with autograft CTy survival (3/3 after 4 days and 3/3 after 7 days). Allograft CTy after 24 h of normothermic HOC and 7 days of hypothermic HOC was rejected. Indicators of oxygen free radical injury were determined:catalase activity was comparable in MTy and CTy (means 14.82 and 6.3-10.8 mm/mg protein, respectively) but superoxide dismutase activity was low in CTy (means 0.01-0.29 and 4.75 U/mg protein, respectively). Malondialdehyde content after 48 h of normothermic HOC was higher in CTy than in MTy (means 2215 and 1275 nmol/g, respectively). The results show that CTy is injured by HOC under conditions tolerated by MTy, and that this difference is related to the greater sensitivity of CTy to oxygen free radical injury.
犬甲状腺组织（CTy）在影响小鼠甲状腺组织（MTy）免疫改变的条件下进行高压氧培养（HOC）。21天后，通过¹²⁵I摄取和组织学确定植入肾包膜下的自体移植物和同种异体移植物的存活情况。与MTy不同，常温HOC（95% O₂，5% CO₂；1.76 kg/cm²）处理48小时的自体移植物CTy未能存活（0/8），而将培养时间缩短至24小时则导致自体移植物CTy存活（3/3）。在低温（5℃）下，HOC可延长至7天，自体移植物CTy存活（4天后3/3，7天后3/3）。常温HOC 24小时和低温HOC 7天后的同种异体移植物CTy被排斥。测定了氧自由基损伤指标：过氧化氢酶活性在MTy和CTy中相当（分别为14.82和6.3 - 10.8 mm/mg蛋白），但CTy中的超氧化物歧化酶活性较低（分别为0.01 - 0.29和4.75 U/mg蛋白）。常温HOC 48小时后，CTy中的丙二醛含量高于MTy（分别为2215和1275 nmol/g）。结果表明，CTy在MTy耐受的条件下受到HOC损伤，这种差异与CTy对氧自由基损伤的更大敏感性有关。
