Kondakova I V, Klisho E V, savenkova O V, Kakurina G V, Choĭnzonov E L, Shishkin D A, Mukhamedov M P
Biomed Khim. 2008 Sep-Oct;54(5):555-60.
The levels of metalloproteinases (MMP-2,-9), their tissue inhibitors (TIMP-1,-2) and extracellular matrix metalloproteinase inducer (EMMPRIN) were studied in tumor tissue and blood serum from patients with head and neck squamous sell carcinoma. Immunohistochemical investigation showed much higher expression of MMP-9 and TIMP-1 in tumor tissue than MMP-2 and TIMP-2. Different expression of the studied parameters (except TIMP-1) in cancer cells and stroma was observed. Enhanced level of MMP-2,-9 and TIMP-2 expression was revealed mainly in fibrocytes, leukocytes and stromal extracellular matrix. Expression of EMMPRIN in tumor cells was higher than in stromal cells. It is possible that carcinoma cells express EMMPRIN to increase MMP production by surrounding cells. Significant decrease of inhibitor expression was observed in cells of carcinomas with N1 grade of metastasis than in tumors without metastasis. Also the decrease in TIMP-1 level was determined in blood serum of patients with metastasis to regional lymph nodes compared with serum of patients without metastasis. Thus, MMP-9 and TIMP-1 play an important role in the development of head and neck squamous sell carcinoma and TIMP-1 level in blood serum and cancer tissues is linked to first grade of regional lymph node metastasis.
对头颈部鳞状细胞癌患者的肿瘤组织和血清中金属蛋白酶（MMP - 2、- 9）、其组织抑制剂（TIMP - 1、- 2）和细胞外基质金属蛋白酶诱导剂（EMMPRIN）的水平进行了研究。免疫组织化学研究显示，肿瘤组织中MMP - 9和TIMP - 1的表达远高于MMP - 2和TIMP - 2。观察到所研究参数（TIMP - 1除外）在癌细胞和基质中的表达存在差异。MMP - 2、- 9和TIMP - 2表达水平的升高主要见于纤维细胞、白细胞和基质细胞外基质。肿瘤细胞中EMMPRIN的表达高于基质细胞。癌细胞可能通过表达EMMPRIN来增加周围细胞的MMP生成。与无转移的肿瘤相比，N1级转移癌的细胞中抑制剂表达显著降低。与无转移患者的血清相比，区域淋巴结转移患者血清中的TIMP - 1水平也有所降低。因此，MMP - 9和TIMP - 1在头颈部鳞状细胞癌的发展中起重要作用，血清和癌组织中的TIMP - 1水平与区域淋巴结转移的一级相关。
