从常规结核病实验室收集的石炭酸复红染色质量的差异。
Variations in quality of carbol fuchsin stains collected from routine tuberculosis laboratories.
作者信息
Zhao Y L, Liu Y H, Jiang G L, Chan W Y, Yip C W, Kam K M
机构信息
National Reference Laboratory, Beijing Chest Disease and Tumour Hospital, Beijing, China.
出版信息
Int J Tuberc Lung Dis. 2009 Jan;13(1):126-9.
SETTING
In-use carbol fuchsin stains were collected from 10 different routine acid-fast bacilli smear microscopy laboratories.
OBJECTIVE
To examine the variations in the composition of carbol fuchsin stains.
METHOD
Carbol fuchsin concentrations were first determined spectrophotometrically by measuring absorbance at 547 nm. High-performance liquid chromatography (HPLC) separated and quantified the four basic fuchsin homologues: para-rosaniline, rosaniline, magenta II and new fuchsin, and identity was confirmed by mass spectrometry (MS).
RESULTS
Absorbance measurement showed that three of 10 (30%) samples contained insufficient carbol fuchsin (<70%). Wide variations in relative proportions of fuchsin homologues were found.
CONCLUSION
The relative abundance of rosaniline + new fuchsin was quite stable among the different laboratories. Spectrophotometry and HPLC/MS are necessary and sensitive tools for monitoring fuchsin quality.
背景
从10个不同的常规抗酸杆菌涂片显微镜检查实验室收集在用的石炭酸复红染液。
目的
检测石炭酸复红染液成分的差异。
方法
首先通过测量547nm处的吸光度用分光光度法测定石炭酸复红的浓度。高效液相色谱法（HPLC）分离并定量四种碱性品红同系物：对玫瑰苯胺、玫瑰苯胺、品红II和新红，并用质谱法（MS）确认其同一性。
结果
吸光度测量显示10个样本中有3个（30%）含有不足的石炭酸复红（<70%）。发现品红同系物的相对比例差异很大。
结论
玫瑰苯胺+新红的相对丰度在不同实验室中相当稳定。分光光度法和HPLC/MS是监测品红质量的必要且灵敏的工具。
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