Norbeck Joakim
Department of Chemical and Biological Engineering/Molecular Biotechnology, Chalmers University of Technology, SE-41296, Göteborg, Sweden.
J Microbiol. 2008 Dec;46(6):692-6. doi: 10.1007/s12275-008-0122-2. Epub 2008 Dec 24.
We have investigated the composition of the conserved Ccr4-Not complex during different physiological states of Saccharomyces cerevisiae. Major changes were found, most notably in the expression of the central scaffold protein Not1p, which was strongly reduced in the absence of glucose. The low expression of Not1p was also evident from the inability of Pop2p to co-purify Not1p in cells from cultures lacking glucose. However, Not1p was still essential under conditions of low expression. The downregulation of Not1p indicates that many of the Ccr4-Not complex components are likely to have roles outside of the complex. We suggest that the use of different carbon sources will be a good starting point to unravel these functions.
我们研究了酿酒酵母不同生理状态下保守的Ccr4-Not复合物的组成。发现了主要变化，最显著的是中心支架蛋白Not1p的表达，在缺乏葡萄糖的情况下其表达大幅降低。从缺乏葡萄糖的培养物中的细胞中Pop2p无法与Not1p共纯化这一点也可明显看出Not1p的低表达。然而，Not1p在低表达条件下仍然是必需的。Not1p的下调表明许多Ccr4-Not复合物组分可能在复合物之外发挥作用。我们认为使用不同的碳源将是揭示这些功能的一个良好起点。
