Whalen R G, Gros F
Biochim Biophys Acta. 1977 Mar 18;475(2):393-402. doi: 10.1016/0005-2787(77)90028-4.
The ability of embryonic chicken muscle initiation factors to translate rabbit globin messenger RNA in an efficient, fractionated cell-free system has been examined. Although muscle factors stimulate leucine incorporation to only 15--35% the levels achieved with rabbit reticulocyte initiation factors, they synthesize more than one globin chain per mRNA molecule and both alpha and beta globin are produced. Increasing the ribosome concentration and adding the polyamine spermidine to the system produce stimulatory effects which are quantitatively and qualitatively similar for both factor preparations. The lower efficiency of synthesis of muscle factors relative to reticulocyte factors is also apparent when mRNA from encephalomyocarditis virus or embryonic chicken muscle polysomes are used in the cell-free system. These results do not support a specific restriction in the capacity of muscle factors to translate globin mRNA. Furthermore, the similarity of the effects of presumed non-specific components on the activity of muscle and reticulocyte factors suggests that globin synthesis in the cell-free system may be controlled in a similar fashion for both preparations.
在一个高效的、分级分离的无细胞系统中，对胚胎鸡肌肉起始因子翻译兔珠蛋白信使核糖核酸（mRNA）的能力进行了检测。尽管肌肉因子刺激亮氨酸掺入的水平仅为兔网织红细胞起始因子所达到水平的15% - 35%，但它们每个mRNA分子能合成不止一条珠蛋白链，并且α和β珠蛋白都能产生。增加核糖体浓度并向系统中添加多胺亚精胺会产生刺激作用，这两种因子制剂在数量和质量上的作用相似。当在无细胞系统中使用脑心肌炎病毒的mRNA或胚胎鸡肌肉多核糖体时，肌肉因子相对于网织红细胞因子的合成效率较低也很明显。这些结果并不支持肌肉因子在翻译珠蛋白mRNA的能力上存在特定限制。此外，假定的非特异性成分对肌肉和网织红细胞因子活性的影响相似，这表明在无细胞系统中，两种制剂的珠蛋白合成可能以类似的方式受到控制。
