Ohara Hirotoshi, Kishi Kazuo, Nakajima Tatsuo
Department of Plastic and Reconstructive Surgery, Keio University, Keio University, Shinjuku-ku Shinanomachi 35, Tokyo, Japan.
Keio J Med. 2008 Dec;57(4):211-6. doi: 10.2302/kjm.57.211.
Rectangular flaps on the rat or mouse dorsum are frequently used for flap survival elongation research. However, since rectangular flaps are purely random, the survival rate varies among individuals. A reliable animal flap model with low individual variation is desirable for flap survival research, especially in the angiogenic field. We investigated the survival rate of paired flaps in the rat dorsum containing 3 vascular territories in each flap, and determined their usefulness for flap elongation research. Two symmetrical adjoining rectangular flaps (11 x 3 cm each) were drawn on the rat dorsum. Two days after material injection, flaps were elevated with only the deep circumflex iliac vessels as the vascular pedicle. Flaps were immediately sutured back and the flap survival area was measured 7 days after the operation. The control group (n = 9) had saline solution injected in both flaps. The bone marrow group (n = 8) had bone marrow injected in the right flap, and saline solution injected in the left flap. In the control group, the survival rate of the paired flaps was not statistically different (right flap, 89.0+/-5.6%; left flap, 89.3+/-4.5%). In the bone marrow group, the survival rate between the bone marrow injected flap (89.9+/-3.7%), and the saline flap (84.8+/-4.3%) was statistically different. The rat dorsal paired island skin flap model shows low difference in flap survival rate and uses an internal control. This is a suitable model for flap survival elongation research.
大鼠或小鼠背部的矩形皮瓣常用于皮瓣存活延长研究。然而，由于矩形皮瓣完全是随机的，个体之间的存活率存在差异。对于皮瓣存活研究，尤其是在血管生成领域，需要一个个体差异小的可靠动物皮瓣模型。我们研究了大鼠背部包含三个血管区域的成对皮瓣的存活率，并确定了它们在皮瓣延长研究中的实用性。在大鼠背部绘制两个对称相邻的矩形皮瓣（每个11×3厘米）。材料注射两天后，仅以旋髂深血管作为血管蒂掀起皮瓣。皮瓣立即缝合回原处，并在术后7天测量皮瓣存活面积。对照组（n = 9）在两个皮瓣中均注射生理盐水。骨髓组（n = 8）在右侧皮瓣中注射骨髓，在左侧皮瓣中注射生理盐水。在对照组中，成对皮瓣的存活率无统计学差异（右侧皮瓣，89.0±5.6%；左侧皮瓣，89.3±4.5%）。在骨髓组中，注射骨髓的皮瓣（89.9±3.7%）和注射生理盐水的皮瓣（84.8±4.3%）之间的存活率有统计学差异。大鼠背部成对岛状皮瓣模型显示皮瓣存活率差异小，并采用了内部对照。这是一个适用于皮瓣存活延长研究的模型。
