Walker K J, Price-Thomas J M, Candlish W, Nicholson R I
Tenovus Institute for Cancer Research, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Br J Cancer. 1991 Oct;64(4):764-8. doi: 10.1038/bjc.1991.395.
Immunohistochemical assays have been employed to study the expression of ER, PgR, EGFR and Ki67 immunostaining in normal breast tissue (n = 76). The expression of ER and PgR was highly variable in both pre and postmenopausal women and was characterised by large numbers of apparently negative cells. This was most evident for ER-ICA staining in tissues removed from premenopausal women. PgR levels were highest in the ducts of premenopausal women, while EGFR expression was elevated in both ducts and lobules. Ki67 expression was observed in less than 10% of all normal cells and was suppressed by the menopause in lobular tissue. Tamoxifen therapy (40 mg d-1) did not influence the expression of PgR, EGFR or Ki67 immunostaining in cancer associated normal tissue (n = 17). A significant increase, however, was observed in the mean percentage ER positivity in ductal tissue. No effect of duration of tamoxifen therapy was observed on the expression of the antigens studied.
免疫组织化学检测已用于研究正常乳腺组织（n = 76）中雌激素受体（ER）、孕激素受体（PgR）、表皮生长因子受体（EGFR）和Ki67免疫染色的表达情况。在绝经前和绝经后女性中，ER和PgR的表达差异很大，其特征是大量细胞明显呈阴性。这在绝经前女性切除组织的ER-ICA染色中最为明显。PgR水平在绝经前女性的导管中最高，而EGFR在导管和小叶中均有升高表达。在所有正常细胞中，Ki67表达率不到10%，且在小叶组织中，绝经后其表达受到抑制。他莫昔芬治疗（40 mg d-1）对癌旁正常组织（n = 17）中PgR、EGFR或Ki67免疫染色的表达没有影响。然而，导管组织中ER阳性的平均百分比显著增加。未观察到他莫昔芬治疗持续时间对所研究抗原表达的影响。
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