El-Shenawy S M A
Dep. of Pharmacology, National Research Centre, Tahrir St., Dokki, Egypt.
Inflammopharmacology. 2009 Apr;17(2):85-92. doi: 10.1007/s10787-008-8034-7.
The present study investigated the effect of ethanol extracts of seeds, pericarp and leaves of Eugenia Jamolana (E. Jamolana) on inflammation, gastric ulcer, anti-oxidants and hepatoprotective in rats. The acute inflammation was induced by intra-plantar injection of carrageenan (100 microl of 1 %) in the rat hind paw. Gastric ulcer was evoked by indomethacin (25 mg/kg) oral administration. Liver damage was induced by given CCL4 (2.5 ml/kg) orally. The median lethal (LD(50)) of the ethanol extract of both seeds and pericarp were determined and revealed that the investigated extracts of seeds and pericarp were non toxic up to 5 g/kg. The anti-inflammatory results showed that the oral administration of ethanol extract of E. Jamolana seeds (250, 500 mg/kg) showed significant inhibition of oedema formation in dose-dependent manner by -27.86, -41.23, -44.73, -51.78 % and by -63.16, -37.77, -47.04, -55.36 % at 1, 2, 3 and 4 h at 1, 2, 3 and 4 h, respectively. While the pericarp given at dose (500 mg/kg) exhibited significant inhibition of the oedema formation by -34.64, -21,8, 19.23 and -33.47 % at 1, 2, 3 and 4 h, respectively post carrageenan injection as compared with saline control group. E. Jamolana leaves fraction 1 given orally at dose of 25 mg/kg, induced non significant change on oedema, while the oedema response was significantly inhibited by -25.14, -33.4, -20.57 and -26.46 % at 1, 2, 3 and 4 h, respectively in group of rats that received leaves fraction 2 at the same dose. Rats were given leaves fraction 3 extract showed inhibition of oedema formation by -4.48 % at 1(st) h post- carrageenan injection, while at 2(nd), 3(rd) and 4(th) h showed non significant change on oedema formation. The acute gastric mucosal lesions was significantly reduced by given ethanol extract of E. Jamolana seeds, pericarp (250, 500 mg/kg) and leaves fractions 1, 2 and 3 (25 mg/kg) respectively in dose dependent manner, as compared with indomethacin treated group (control group). All tested extracts showed significant reduction in elevated serum ALT, AST and ALP levels as compared with CCl4 treated group. The ethanol extract of E. Jamolana seeds, pericarp and leaves fractions 1, 2, 3 showed significant elevation of blood GSH level and significant reduction in elevated plasma lipid peroxides (MDA) as compared with CCl4 treated group. In conclusion we can see that the ethanol extracts of E. Jamolana of seeds, pericarp and leaves fractions showed anti-inflammatory, anti-ulcer, hepatoprotective and anti-oxidants activity.
本研究调查了番樱桃种子、果皮和叶子的乙醇提取物对大鼠炎症、胃溃疡、抗氧化剂及肝脏保护作用的影响。通过在大鼠后爪足底注射角叉菜胶（1%的100微升）诱导急性炎症。通过口服吲哚美辛（25毫克/千克）诱发胃溃疡。通过口服给予四氯化碳（2.5毫升/千克）诱导肝损伤。测定了种子和果皮乙醇提取物的半数致死量（LD(50)），结果显示所研究的种子和果皮提取物在高达5克/千克的剂量下无毒。抗炎结果表明，口服番樱桃种子乙醇提取物（250、500毫克/千克）在1、2、3和4小时分别以剂量依赖性方式显著抑制水肿形成，抑制率分别为-27.86%、-41.23%、-44.73%、-51.78%和-63.16%、-37.77%、-47.04%、-55.36%。与生理盐水对照组相比，在注射角叉菜胶后1、2、3和4小时，给予剂量为500毫克/千克的果皮分别显著抑制水肿形成，抑制率为-34.64%、-21.8%、19.23%和-33.47%。口服给予剂量为25毫克/千克的番樱桃叶子组分1对水肿诱导无显著变化，而在接受相同剂量叶子组分2的大鼠组中，在1、2、3和4小时水肿反应分别显著抑制-25.14%、-33.4%、-20.57%和-26.46%。给予番樱桃叶子组分3提取物的大鼠在注射角叉菜胶后第1小时显示水肿形成抑制率为-4.48%，而在第2、3和4小时水肿形成无显著变化。与吲哚美辛治疗组（对照组）相比，分别给予番樱桃种子、果皮（250、500毫克/千克）和叶子组分1、2和3（25毫克/千克）的乙醇提取物可显著减轻急性胃黏膜损伤，且呈剂量依赖性。与四氯化碳治疗组相比，所有测试提取物均显著降低血清谷丙转氨酶（ALT）、谷草转氨酶（AST）和碱性磷酸酶（ALP）水平的升高。与四氯化碳治疗组相比，番樱桃种子、果皮和叶子组分1、2、3的乙醇提取物显著提高血液中谷胱甘肽（GSH）水平，并显著降低血浆脂质过氧化物（MDA）升高水平。总之，我们可以看到番樱桃种子、果皮和叶子组分的乙醇提取物具有抗炎、抗溃疡、肝脏保护和抗氧化活性。
