肾衰宁分散片对慢性肾衰竭大鼠肾脏中一氧化氮、一氧化氮合酶、超氧化物歧化酶及丙二醛水平的影响

[Effect of shenshuaining dispersible tablets on the levels of NO, NOS, SOD and MDA in kidney of chronic renal failure rats].

作者信息

Chen Ying, Xiao Wei, Ma Yun, Hou Lian-bing

机构信息

Department of Pharmacy, Nanfang Hospital, China.

出版信息

Zhong Yao Cai. 2008 Aug;31(8):1190-3.

PMID:19112899

Abstract

OBJECTIVE

To observe the effect of Shenshuaining dispersible tablets on the levels of NO, NOS, SOD and MDA in kidney of chronic renal failure (CRF) rats induced by adenine.

METHODS

Seventy-two Sprague-Dawley rats were randomly divided into 6 groups: normal control group, model control group, Niaoduqing group, Shenshuaining dispersible tablets group(high, middle and low dose). From the second to the sixth group, the rats were prepared for model of CRF by being fed 0.5% adenine. After giving corresponding drugs for 7 weeks, we detected the levels of nitric oxide (NO), total nitric oxide synthase (T-NOS), inducing nitric oxide synthase (iNOS), configuration nitric oxide synthase (cNOS), total superoxide dismutase (SOD), Cu/Zn-SOD and malonaldehyde (MDA) in every rat's kidney tissue.

RESULTS

Compared with normal rats, CRF rats' renal tissue's levels of NO, T-NOS, cNOS, T-SOD and Cu/Zn-SOD markedly decreased. Both the iNOS and MDA contents significantly increased. Shenshuaining dispersible tablets could markedly ameliorate above indexes.

CONCLUSION

Shenshuaining dispersible tablets can effectively improve CRF rats' antioxidation effect and reduce damnification of free radical and protect the kidney.

摘要

目的

观察肾衰宁分散片对腺嘌呤诱导的慢性肾衰竭（CRF）大鼠肾脏中一氧化氮（NO）、一氧化氮合酶（NOS）、超氧化物歧化酶（SOD）和丙二醛（MDA）水平的影响。

方法

将72只Sprague-Dawley大鼠随机分为6组：正常对照组、模型对照组、尿毒清组、肾衰宁分散片组（高、中、低剂量）。从第二组至第六组，通过喂饲0.5%腺嘌呤制备CRF模型。给予相应药物7周后，检测每只大鼠肾脏组织中一氧化氮（NO）、总一氧化氮合酶（T-NOS）、诱导型一氧化氮合酶（iNOS）、结构型一氧化氮合酶（cNOS）、总超氧化物歧化酶（SOD）、铜/锌超氧化物歧化酶（Cu/Zn-SOD）和丙二醛（MDA）的水平。