Wei Feng-xiang, Li Mei-yu, Song Yu-hong, Li Hong-zhi
Department of biology, Guangdong pharmaceutical university, Guangzhou 510006, China.
Zhong Yao Cai. 2008 Aug;31(8):1197-200.
To study the effects of essential oil extracted from pine needles on HepG2 cell line.
HepG2 cells were treated with essential oil extracted from pine needles. Cell growth rate was determined with MTF assay, cell morphologic changes were examined under transmission electromicroscope and HE straining. Flow cytometry was used to exmine apoptotic cells. Bcl-2 gene expression was determined by flow cytometry and telomerase activity by TRAP assay.
Essential oils from pine needles could not only repress the growth of HepG2 cells significantly, but also induce apoptosis to them. Both dose-effect and time-effect relationship could be confirmed. Typical morphology changes of apoptosis such as nuclear enrichment and karyorrhexis were observed through transmission electromicroscope and HE straining. Telomerase activity was down regulated in the essential oil extracted from pine needles induced apoptotic cells. The expression of bcl-2 gene was suppressed after the essential oil from pine needles treatement.
The essential oil extracted from pine needles can inhibit cell growth of HepG2 cell line and induce apoptosis, which may associate with inhibition of telomerase activity and bcl-2 may be involved in the regulation of telomerase activity.
研究从松针中提取的挥发油对肝癌细胞系HepG2的作用。
用从松针中提取的挥发油处理HepG2细胞。采用MTT法测定细胞生长率，通过透射电镜和苏木精-伊红染色观察细胞形态学变化。用流式细胞术检测凋亡细胞。采用流式细胞术检测Bcl-2基因表达，用端粒重复序列扩增法检测端粒酶活性。
松针挥发油不仅能显著抑制HepG2细胞生长，还能诱导其凋亡。剂量效应和时间效应关系均得到证实。通过透射电镜和苏木精-伊红染色观察到典型的凋亡形态学变化，如核浓缩和核碎裂。在松针挥发油诱导凋亡的细胞中端粒酶活性下调。松针挥发油处理后Bcl-2基因表达受到抑制。
从松针中提取的挥发油可抑制HepG2细胞系的细胞生长并诱导凋亡，这可能与端粒酶活性的抑制有关，且Bcl-2可能参与端粒酶活性的调节。
