Chang Ying-Jun, Zhao Xiang-Yu, Huo Ming-Rui, Luo Xiao-Hua, Huang Xiao-Jun
Peking University Institute of Hematology & Peking University People's Hospital, Beijing 100044, China.
Zhonghua Xue Ye Xue Za Zhi. 2008 Aug;29(8):512-6.
To investigate the cell composition in granulocyte colony-stimulating factor One (rhG-CSF) primed bone marrow grafts (G-BM) from donors with different characteristics.
hundred and fifty healthy donors were injected subcutaneously rhG-CSF (5 microg x kg(-1) x d(-1)) in five consecutive days. Bone marrow and peripheral blood stem cells were harvested at day 4 and day 5. The number of CD3, CD3+ CD4+, CD3+ CD8+, CD14+ , CD34+ and CD3+ CD4- CD8- cells were determined by multicolor flow cytometry. Cell composition of G-BM from donors with different characteristics, including sex, age, weight, pregnancy were analysed.
The absolute number of nuclear cells (NCs), lymphocytes, CD3+, CD3+ CD4+, CD3+ CD8+, CD14+, CD34+ and CD3+ CD4- CD8- cells in G-BM were 31 050 (12 200 -58 100), 2122 (506 - 6618), 1344 (307 - 4791), 692 (145 - 3038), 616 (112 - 2575), 986 (265 -2958), 63 (11 -505) and 83 (9 -390) per microliter, respectively. The number of NCs [33 800 (18 600 - 57 100)], CD34+ cells [76 (22 -505)], and CD3+ CD4+ CD8- regulatory T cells [97 (11 - 380)] harvested from younger donors (aged < or = 38 years) were significantly higher than those from older ones (aged > 38) [28000 (12200-58100), 49 (11-220), and 65 (9 - 389) per microliter (P = 0.027, < 0.001 and = 0.001, respectively)]. Compared with that in donors with peripheral blood monocytes (PBMs) < or = 0.37 x 10(9)/L, higher number of NCs in G-BM [27 150 (13 800 - 58 100) vs 33 550 (12 200 - 57 100), P = 0.005] were collected in donors with PBMs > 0.37 x 10(9)/L. Multivariate analysis showed that donors age (< or = 38 vs > 38 years; P = 0.01) and monocyte number in peripheral blood (< or = 0.37 x 10(9)/L vs > 0.37 x 10(9)/L; P = 0. 003) were factors predicting for NC yields, and donors age ( < or = 38 vs > 38 years; P < 0.001) was factor predicting for yields of CD34+ cells (P < 0.001) and CD3+ CD4- CD8- regulatory T cells (P = 0.001) collection.
Donor age is a factor for the yields of NCs, CD34+ cells, and CD3+ CD4- CD8- regulatory T cells collection in G-BM, and donor's PBMs more than 0.37 x 10(9)/L, is another factor affecting NCs harvests.
研究不同特征供者的粒细胞集落刺激因子（rhG-CSF）动员的骨髓移植物（G-BM）中的细胞组成。
150名健康供者连续5天皮下注射rhG-CSF（5μg·kg⁻¹·d⁻¹）。在第4天和第5天采集骨髓和外周血干细胞。通过多色流式细胞术测定CD3、CD3⁺CD4⁺、CD3⁺CD8⁺、CD14⁺、CD34⁺和CD3⁺CD4⁻CD8⁻细胞的数量。分析不同特征供者（包括性别、年龄、体重、妊娠情况）的G-BM的细胞组成。
G-BM中核细胞（NCs）、淋巴细胞、CD3⁺、CD3⁺CD4⁺、CD3⁺CD8⁺、CD14⁺、CD34⁺和CD3⁺CD4⁻CD8⁻细胞的绝对数量分别为每微升31050（12200 - 58100）、2122（506 - 6618）、1344（307 - 4791）、692（145 - 3038）、616（112 - 2575）、986（265 - 2958）、63（11 - 505）和83（9 - 390）。从较年轻供者（年龄≤38岁）采集的NCs[33800（18600 - 57100）]、CD34⁺细胞[76（22 - 505）]和CD3⁺CD4⁺CD8⁻调节性T细胞[97（11 - 380）]的数量显著高于较年长供者（年龄>38岁）[每微升28000（12200 - 58100）、49（11 - 220）和65（9 - 389），P分别为0.027、<0.001和 = 0.001]。与外周血单核细胞（PBMs）≤0.37×10⁹/L的供者相比，PBMs>0.37×10⁹/L的供者的G-BM中NCs数量更高[27150（13800 - 58100）对33550（12200 - 57100），P = 0.005]。多因素分析显示，供者年龄（≤38岁对>38岁；P = 0.01）和外周血单核细胞数量（≤0.37×10⁹/L对>0.37×10⁹/L；P = 0.003）是预测NC产量的因素，供者年龄（≤38岁对>38岁；P<0.001）是预测CD34⁺细胞（P<0.001）和CD3⁺CD4⁻CD8⁻调节性T细胞（P = 0.001）采集产量的因素。
供者年龄是G-BM中NCs、CD34⁺细胞和CD3⁺CD4⁻CD8⁻调节性T细胞采集产量的一个因素，供者PBMs超过0.37×10⁹/L是影响NCs收获的另一个因素。
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